BMRB Entry 1702
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR1702
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Title: Positioning of a Spin-Labeled Substrate Analogue into the Structure of delta5-3-Ketosteroid Isomerase by Combined Kinetic, Magnetic Resonance, and X-ray Diffraction Methods
Deposition date: 1995-07-31 Original release date: 1999-06-14
Authors: Kuliopulos, Anthan; Westbrook, Edwin; Talalay, Paul; Mildvan, Albert
Citation: Kuliopulos, Anthan; Westbrook, Edwin; Talalay, Paul; Mildvan, Albert. "Positioning of a Spin-Labeled Substrate Analogue into the Structure of delta5-3-Ketosteroid Isomerase by Combined Kinetic, Magnetic Resonance, and X-ray Diffraction Methods" Biochemistry 26, 3927-3937 (1987).
Assembly members:
delta5-3-ketosteroid isomerase, polymer, 125 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: not available
Entity Sequences (FASTA):
delta5-3-ketosteroid isomerase: MNTPEHMTAVVQRYVAALNA
GNLNGIVALFADNATVEDPV
GSEPRSGTAAIREFYANSLK
LPLAVELTQEVRAVANQAAF
AFTVSFEYQGRKTVVAPIDH
FRFNGAGKVVSMRALFGEKN
IHAGA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 32 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | delta5-3-ketosteroid isomerase | 1 |
Entities:
Entity 1, delta5-3-ketosteroid isomerase 125 residues - Formula weight is not available
| 1 | MET | ASN | THR | PRO | GLU | HIS | MET | THR | ALA | VAL | ||||
| 2 | VAL | GLN | ARG | TYR | VAL | ALA | ALA | LEU | ASN | ALA | ||||
| 3 | GLY | ASN | LEU | ASN | GLY | ILE | VAL | ALA | LEU | PHE | ||||
| 4 | ALA | ASP | ASN | ALA | THR | VAL | GLU | ASP | PRO | VAL | ||||
| 5 | GLY | SER | GLU | PRO | ARG | SER | GLY | THR | ALA | ALA | ||||
| 6 | ILE | ARG | GLU | PHE | TYR | ALA | ASN | SER | LEU | LYS | ||||
| 7 | LEU | PRO | LEU | ALA | VAL | GLU | LEU | THR | GLN | GLU | ||||
| 8 | VAL | ARG | ALA | VAL | ALA | ASN | GLN | ALA | ALA | PHE | ||||
| 9 | ALA | PHE | THR | VAL | SER | PHE | GLU | TYR | GLN | GLY | ||||
| 10 | ARG | LYS | THR | VAL | VAL | ALA | PRO | ILE | ASP | HIS | ||||
| 11 | PHE | ARG | PHE | ASN | GLY | ALA | GLY | LYS | VAL | VAL | ||||
| 12 | SER | MET | ARG | ALA | LEU | PHE | GLY | GLU | LYS | ASN | ||||
| 13 | ILE | HIS | ALA | GLY | ALA |
Samples:
sample_one:
sample_condition_set_one: pH: 6.8 na; temperature: 297 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz