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PDB ID: 2kzy
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17023
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
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Citation: Garner, Thomas; Strachan, Joanna; Long, Jed; Layfield, Robert; Searle, Mark. "Co-localisation of ubiquitin receptors ZNF216 and p62 in a ubiquitin-mediated ternary complex" Nat. Struct. Biol. ., .-..
Assembly members:
ZNF216-A20, polymer, 62 residues, 6529.2 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PGEX-4T1
Entity Sequences (FASTA):
ZNF216-A20: GSMAQETNQTPGPMLCSTGC
GFYGNPRTNGMCSVCYKEHL
QRQQNSGRMSPMGTASGSNS
PT
Data type | Count |
13C chemical shifts | 207 |
15N chemical shifts | 65 |
1H chemical shifts | 276 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Znf_A20 | 1 |
2 | Zinc | 2 |
Entity 1, Znf_A20 62 residues - 6529.2 Da.
Residues G1 amd S2 are non native artifacts from cloning
1 | GLY | SER | MET | ALA | GLN | GLU | THR | ASN | GLN | THR | ||||
2 | PRO | GLY | PRO | MET | LEU | CYS | SER | THR | GLY | CYS | ||||
3 | GLY | PHE | TYR | GLY | ASN | PRO | ARG | THR | ASN | GLY | ||||
4 | MET | CYS | SER | VAL | CYS | TYR | LYS | GLU | HIS | LEU | ||||
5 | GLN | ARG | GLN | GLN | ASN | SER | GLY | ARG | MET | SER | ||||
6 | PRO | MET | GLY | THR | ALA | SER | GLY | SER | ASN | SER | ||||
7 | PRO | THR |
Entity 2, Zinc - Zn - 65.409 Da.
1 | ZN |
BMRB | 17024 |
PDB | 2KZY 2L00 |
DBJ | BAC36321 BAF83538 BAG73932 |
GB | AAC42600 AAC42601 AAC42602 AAC61801 AAH11018 |
REF | NP_001090975 NP_001094515 NP_001095890 NP_001095891 NP_001157077 |
SP | B5DF11 O76080 O88878 |
TPG | DAA26877 |
AlphaFold | B5DF11 O76080 O88878 |
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