BMRB Entry 17023

Title:
Solution NMR structure of the ZNF216 A20 zinc finger
Deposition date:
2010-06-28
Original release date:
2012-08-02
Authors:
Garner, Thomas; Long, Jed; Searle, Mark; Layfield, Robert
Citation:

Citation: Garner, Thomas; Strachan, Joanna; Long, Jed; Layfield, Robert; Searle, Mark. "Co-localisation of ubiquitin receptors ZNF216 and p62 in a ubiquitin-mediated ternary complex"  Nat. Struct. Biol. ., .-..

Assembly members:

Assembly members:
ZNF216-A20, polymer, 62 residues, 6529.2 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PGEX-4T1

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts207
15N chemical shifts65
1H chemical shifts276

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Znf_A201
2Zinc2

Entities:

Entity 1, Znf_A20 62 residues - 6529.2 Da.

Residues G1 amd S2 are non native artifacts from cloning

1   GLYSERMETALAGLNGLUTHRASNGLNTHR
2   PROGLYPROMETLEUCYSSERTHRGLYCYS
3   GLYPHETYRGLYASNPROARGTHRASNGLY
4   METCYSSERVALCYSTYRLYSGLUHISLEU
5   GLNARGGLNGLNASNSERGLYARGMETSER
6   PROMETGLYTHRALASERGLYSERASNSER
7   PROTHR

Entity 2, Zinc - Zn - 65.409 Da.

1   ZN

Related Database Links:

BMRB 17024
PDB 2KZY 2L00
DBJ BAC36321 BAF83538 BAG73932
GB AAC42600 AAC42601 AAC42602 AAC61801 AAH11018
REF NP_001090975 NP_001094515 NP_001095890 NP_001095891 NP_001157077
SP B5DF11 O76080 O88878
TPG DAA26877
AlphaFold B5DF11 O76080 O88878

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks