BMRB Entry 17059

Name: Identification of a novel ubiquitin binding site of STAM1 VHS domain by NMR spectroscopy
Published: 2010-09-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17059

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Identification of a novel ubiquitin binding site of STAM1 VHS domain by NMR spectroscopy

Deposition date:

2010-07-13

Original release date:

2010-09-08

Authors:

Hong, Yoon-Hun; Ahn, Hee-Chul; Lim, Jongsoo; Kim, Hong-Man; Ji, Hye-Young; Lee, Seunga; Kim, Ji-Hun; Park, Eun; Song, Hyun; Lee, Bong-Jin

Citation:

Citation: Hong, Yoon-Hun; Ahn, Hee-Chul; Lim, Jongsoo; Kim, Hong-Man; Ji, Hye-Young; Lee, Seunga; Kim, Ji-Hun; Park, Eun; Song, Hyun; Lee, Bong-Jin. "Identification of a novel ubiquitin binding site of STAM1 VHS domain by NMR spectroscopy" FEBS Lett. 583, 287-292 (2009).

Assembly members:

Assembly members:
STAM1, polymer, 178 residues, Formula weight is not available
ubiquitin, polymer, 156 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-glutathionse S-transferase

Entity Sequences (FASTA):

Entity Sequences (FASTA):
STAM1: PLFATNPFDQDVEKATSEMN TAEDWGLILDICDKVGQSRT GPKDCLRSIMRRVNHKDPHV AMQALTLLGACVSNCGKIFH LEVCSRDFASEVSNVLNKGH PKVCEKLKALMVEWTDEFKN DPQLSLISAMIKNLKEQGVT FPAIGSQAAEQAKASPALVA KDPGTVANKKEEEDLAKA
ubiquitin: MQIFVKTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGGAKKR KKKSYTTPKKNKHKRKKVKL AVLKYYKVDENGKISRLRRE CPSDECGAGVFMASHFDRHY CGKCCLTYCFNKPEDK

Data sets:

binding_constants

Data type	Count
binding constants	4

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	STAM1	1
2	ubiquitin	2

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1

Related Database Links:

BMRB	16763 17065
PDB	3LDZ 3J3A 4CXC 4KZX 4KZY 4KZZ 4UQ4 4UQ5 4W23 4W28
DBJ	BAE26170 BAE28168 BAE34187 BAE37536 BAE40949 BAA04129 BAA09096 BAA11843 BAB27296 BAB31357
GB	AAC50734 AAC52840 AAH30586 AAH44666 AAH55326 AAA28997 AAA36788 AAA53067 AAA57047 AAB03872
REF	NP_001070310 NP_001102591 NP_003464 NP_035614 XP_001094352 NP_001013290 NP_001016172 NP_001029037 NP_001086065 NP_001129064
SP	P70297 Q92783 P0CG50 P0CG69 P62978 P62979 P62982
TPG	DAA23567 DAA24675
EMBL	CAA44911 CAA57432 CAG12343 CAI73380 CAJ83451
PIR	UQHUR7
AlphaFold	P70297 Q92783 P0CG50 P0CG69 P62978 P62979 P62982

BMRB Entry 17059

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links: