BMRB Entry 17100
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17100
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Title: Backbone Dynamics of the C-terminal SH2 Domain of the p85alpha Subunit of Phosphoinositide 3-kinase: Effect of Phosphotyrosine-Peptide Binding and Characterization of Slow Conformational Exchange Processes PubMed: 10835283
Deposition date: 2010-08-02 Original release date: 2010-11-10
Authors: Kristensen, Soren; Siegal, Gregg; Sankar, Andrew; Driscoll, Paul
Citation: Kristensen, Soren; Siegal, Gregg; Sankar, Andrew; Driscoll, Paul. "Backbone Dynamics of the C-terminal SH2 Domain of the p85alpha Subunit of Phosphoinositide 3-Kinase: Effect of Slow Conformational Exchange Processes" J. Mol. Biol. 299, 771-788 (2000).
Assembly members:
P85ALPHA, polymer, 112 residues, Formula weight is not available
PTR, non-polymer, 261.168 Da.
Natural source: Common Name: Cattle Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: na
Entity Sequences (FASTA):
P85ALPHA: MEDLPHHDEKTWNVGSSNRN
KAENLLRGKRDGTFLVRESS
KQGCYACSVVVDGEVKHCVI
NKTATGYGFAEPYNLYSSLK
ELVLHYQHTSLVQHNDSLNV
TLAYPVYAQQRR
- chemical_rates
| Data type | Count |
| kinetic rates | 2 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | P85ALPHA | 1 |
| 2 | pY | 2 |
Entities:
Entity 1, P85ALPHA 112 residues - Formula weight is not available
| 1 | MET | GLU | ASP | LEU | PRO | HIS | HIS | ASP | GLU | LYS | ||||
| 2 | THR | TRP | ASN | VAL | GLY | SER | SER | ASN | ARG | ASN | ||||
| 3 | LYS | ALA | GLU | ASN | LEU | LEU | ARG | GLY | LYS | ARG | ||||
| 4 | ASP | GLY | THR | PHE | LEU | VAL | ARG | GLU | SER | SER | ||||
| 5 | LYS | GLN | GLY | CYS | TYR | ALA | CYS | SER | VAL | VAL | ||||
| 6 | VAL | ASP | GLY | GLU | VAL | LYS | HIS | CYS | VAL | ILE | ||||
| 7 | ASN | LYS | THR | ALA | THR | GLY | TYR | GLY | PHE | ALA | ||||
| 8 | GLU | PRO | TYR | ASN | LEU | TYR | SER | SER | LEU | LYS | ||||
| 9 | GLU | LEU | VAL | LEU | HIS | TYR | GLN | HIS | THR | SER | ||||
| 10 | LEU | VAL | GLN | HIS | ASN | ASP | SER | LEU | ASN | VAL | ||||
| 11 | THR | LEU | ALA | TYR | PRO | VAL | TYR | ALA | GLN | GLN | ||||
| 12 | ARG | ARG |
Entity 2, pY - C9 H12 N O6 P - 261.168 Da.
| 1 | PTR |
Samples:
sample_1: perdeuterated Tris 50 mM; sodium chloride 50 mM; EDTA 1 mM; beta-mercaptoethanol 1 mM; P85ALPHA 1.0 mM; pY 1.5 molar equivalents; H2O 90%; D2O 10%
sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 290 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- na na na MHz