BMRB Entry 17110

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PDB ID: 2l21
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17110
MolProbity Validation Chart

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Title:
chicken IGF2R domain 11
Deposition date:
2010-08-10
Original release date:
2012-08-30
Authors:
Williams, Christopher; Hoppe, Hans; Strickland, Madeline; Frago, Susana; Ellis, Rosamund; Wattana-Amorn, Pakorn; Prince, Stuart; Zaccheo, Oliver; Forbes, Briony; Jones, Yvonne; Rezgui, Dellel; Crump, Matthew; Hassan, Bassim
Citation:

Citation: Williams, Christopher; Hoppe, Hans; Rezgui, Dellel; Strickland, Madeline; Frago, Susana; Ellis, Rosamund; Wattana-Amorn, Pakorn; Prince, Stuart; Zaccheo, Oliver; Forbes, Briony; Jones, E.; Crump, Matthew; Hassan, A.; Grutzner, Frank; Nolan, Catherine; Mungall, Andrew. "An exon splice enhancer primes IGF2:IGF2R binding site structure and function evolution"  Science 338, 1209-1213 (2012).
PubMed: 23197533

Assembly members:

Assembly members:
IGF2R, polymer, 154 residues, 16408.426 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET26a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IGF2R: MKSNVQNDCRVTNPATGHLF DLTSLKRESGYTITDSHNRK IELNVCAEAKSSCANGAAVC ITDGPKTLNAGKLSKTLTYE DQVLKLVYEDGDPCPTDLKM KHKSYFSFVCKSDAGDDSQP VFLSFDEQTCTSYFSWHTSL ACEEEVPRHHHHHH

Data sets:
Data typeCount
13C chemical shifts592
15N chemical shifts154
1H chemical shifts959

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1IGF2R1

Entities:

Entity 1, IGF2R 154 residues - 16408.426 Da.

Domain 11 of the IGF2R from Gallus gallus (chicken). The C-terminal residues EEVPRHHHHHH are a non-native affinity tag

1   METLYSSERASNVALGLNASNASPCYSARG
2   VALTHRASNPROALATHRGLYHISLEUPHE
3   ASPLEUTHRSERLEULYSARGGLUSERGLY
4   TYRTHRILETHRASPSERHISASNARGLYS
5   ILEGLULEUASNVALCYSALAGLUALALYS
6   SERSERCYSALAASNGLYALAALAVALCYS
7   ILETHRASPGLYPROLYSTHRLEUASNALA
8   GLYLYSLEUSERLYSTHRLEUTHRTYRGLU
9   ASPGLNVALLEULYSLEUVALTYRGLUASP
10   GLYASPPROCYSPROTHRASPLEULYSMET
11   LYSHISLYSSERTYRPHESERPHEVALCYS
12   LYSSERASPALAGLYASPASPSERGLNPRO
13   VALPHELEUSERPHEASPGLUGLNTHRCYS
14   THRSERTYRPHESERTRPHISTHRSERLEU
15   ALACYSGLUGLUGLUVALPROARGHISHIS
16   HISHISHISHIS

Related Database Links:

PDB 2L21

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