BMRB Entry 17112
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17112
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Title: 1H, 13C and 15N NMR Assignments of the C1B Subdomains of PKC-delta. PubMed: 21132404
Deposition date: 2010-08-10 Original release date: 2010-12-08
Authors: Ziemba, Brian; Jones, David; Booth, Jamie
Citation: Ziemba, Brian; Booth, Jamie; Jones, David. "1H, 13C and 15N NMR assignments of the C1A and C1B subdomains of PKC-delta." Biomol. NMR Assignments 5, 125-129 (2011).
Assembly members:
PKC_delta_C1B, polymer, 82 residues, 9408.8 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T1
Entity Sequences (FASTA):
PKC_delta_C1B: GSPEFIFQKERFNIDMPHRF
KVYNYMSPTFCDHCGSLLWG
LVKQGLKCEDCGMNVHHKCR
EKVANLCGINQKVDSSGRIV
TD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 319 |
| 15N chemical shifts | 73 |
| 1H chemical shifts | 441 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | C1B construct | 1 |
| 2 | Zinc 1 | 2 |
| 3 | Zinc 2 | 2 |
Entities:
Entity 1, C1B construct 82 residues - 9408.8 Da.
Residues 214-218 and 286-295 are derived from pGEX-4T1 vector. Residues 219-285 are from PKC-delta, and 231-280 is the 50 residue C1B domain.
| 1 | GLY | SER | PRO | GLU | PHE | ILE | PHE | GLN | LYS | GLU | ||||
| 2 | ARG | PHE | ASN | ILE | ASP | MET | PRO | HIS | ARG | PHE | ||||
| 3 | LYS | VAL | TYR | ASN | TYR | MET | SER | PRO | THR | PHE | ||||
| 4 | CYS | ASP | HIS | CYS | GLY | SER | LEU | LEU | TRP | GLY | ||||
| 5 | LEU | VAL | LYS | GLN | GLY | LEU | LYS | CYS | GLU | ASP | ||||
| 6 | CYS | GLY | MET | ASN | VAL | HIS | HIS | LYS | CYS | ARG | ||||
| 7 | GLU | LYS | VAL | ALA | ASN | LEU | CYS | GLY | ILE | ASN | ||||
| 8 | GLN | LYS | VAL | ASP | SER | SER | GLY | ARG | ILE | VAL | ||||
| 9 | THR | ASP |
Entity 2, Zinc 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: PKC_delta_C1B, [U-98% 15N; U-99% 13C], 300 uM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 1 mM; zinc chloride 10 uM; D2O, [U-100% 2H], 10%; H2O, [U-100% 2H], 90%
sample_2: PKC_delta_C1B, [U-98% 15N; U-99% 13C], 300 uM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 1 mM; zinc chloride 10 uM; D2O, [U-100% 2H], 100%
sample_conditions_1: pH: 6.9; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 2D (HB)CB(CGCD)HD | sample_2 | isotropic | sample_conditions_1 |
| 2D (HB)CB(CGCDCE)HE | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRDraw v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing
ANALYSIS v1.0, CCPN - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Varian INOVA 900 MHz
- Varian INOVA 600 MHz
Related Database Links:
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts