BMRB Entry 17121

Name: Folding Kinetics of the SH3 Domain of PI3 Kinase by Real-time NMR Combined with Optical Spectroscopy
Published: 2010-11-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17121

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Folding Kinetics of the SH3 Domain of PI3 Kinase by Real-time NMR Combined with Optical Spectroscopy

Deposition date:

2010-08-11

Original release date:

2010-11-10

Authors:

Guijarro, J.; Morton, Craig; Plaxco, Kevin; Campbell, Iain; Dobson, Christopher

Citation:

Citation: Guijarro, J.; Morton, Craig; Plaxco, Kevin; Campbell, Iain; Dobson, Christopher. "Folding Kinetics of the SH3 Domain of PI3 Kinase by Real-time NMR Combined with Optical Spectroscopy" J. Mol. Biol. 276, 657-667 (1998).
PubMed: 9551103

Assembly members:

Assembly members:
PISH3, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: na

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PISH3: GSMSAEGYQYRALYDYKKER EEDIDLHLGDILTVNKGSLV ALGFSDGQEARPEEIGWLNG YNETTGERGDFPGTYVEYIG RKKISPWNSS

Data sets:

chemical_rates
- Chemical_rate_list_1
- Chemical_rate_list_2

Data type	Count
kinetic rates	4

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PISH3	1

Entities:

Entity 1, PISH3 90 residues - Formula weight is not available

84 residues from PI3-SH3 domain + 2 residue N terminal addition (GS) and 4 residue C terminal addition (WNSS)

1	GLY	SER	MET	SER	ALA	GLU	GLY	TYR	GLN	TYR
2	ARG	ALA	LEU	TYR	ASP	TYR	LYS	LYS	GLU	ARG
3	GLU	GLU	ASP	ILE	ASP	LEU	HIS	LEU	GLY	ASP
4	ILE	LEU	THR	VAL	ASN	LYS	GLY	SER	LEU	VAL
5	ALA	LEU	GLY	PHE	SER	ASP	GLY	GLN	GLU	ALA
6	ARG	PRO	GLU	GLU	ILE	GLY	TRP	LEU	ASN	GLY
7	TYR	ASN	GLU	THR	THR	GLY	GLU	ARG	GLY	ASP
8	PHE	PRO	GLY	THR	TYR	VAL	GLU	TYR	ILE	GLY
9	ARG	LYS	LYS	ILE	SER	PRO	TRP	ASN	SER	SER

Name	Sample	Sample state	Sample conditions
1H NMR	sample_1	isotropic	sample_conditions_1
1H NMR	sample_1	isotropic	sample_conditions_2

BMRB	16448 19110
PDB	1PHT 1PKS 1PKT 1PNJ 2PNI 3I5R 3I5S
DBJ	BAA18932
EMBL	CAH92731
GB	AAA79511 AAC52847 AAH26146 AAH94795 ABK42316
PRF	2210313A
REF	NP_001070963 NP_001126593 NP_001248126 NP_037137 NP_777000
SP	P23727 P26450 P27986 Q5R685 Q63787
TPG	DAA17994
AlphaFold	P23727 P26450 P27986 Q5R685 Q63787

BMRB Entry 17121

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	SER	MET	SER	ALA	GLU	GLY	TYR	GLN	TYR
2	ARG	ALA	LEU	TYR	ASP	TYR	LYS	LYS	GLU	ARG
3	GLU	GLU	ASP	ILE	ASP	LEU	HIS	LEU	GLY	ASP
4	ILE	LEU	THR	VAL	ASN	LYS	GLY	SER	LEU	VAL
5	ALA	LEU	GLY	PHE	SER	ASP	GLY	GLN	GLU	ALA
6	ARG	PRO	GLU	GLU	ILE	GLY	TRP	LEU	ASN	GLY
7	TYR	ASN	GLU	THR	THR	GLY	GLU	ARG	GLY	ASP
8	PHE	PRO	GLY	THR	TYR	VAL	GLU	TYR	ILE	GLY
9	ARG	LYS	LYS	ILE	SER	PRO	TRP	ASN	SER	SER

1	GLY	SER	MET	SER	ALA	GLU	GLY	TYR	GLN	TYR
2	ARG	ALA	LEU	TYR	ASP	TYR	LYS	LYS	GLU	ARG
3	GLU	GLU	ASP	ILE	ASP	LEU	HIS	LEU	GLY	ASP
4	ILE	LEU	THR	VAL	ASN	LYS	GLY	SER	LEU	VAL
5	ALA	LEU	GLY	PHE	SER	ASP	GLY	GLN	GLU	ALA
6	ARG	PRO	GLU	GLU	ILE	GLY	TRP	LEU	ASN	GLY
7	TYR	ASN	GLU	THR	THR	GLY	GLU	ARG	GLY	ASP
8	PHE	PRO	GLY	THR	TYR	VAL	GLU	TYR	ILE	GLY
9	ARG	LYS	LYS	ILE	SER	PRO	TRP	ASN	SER	SER

1	GLY	SER	MET	SER	ALA	GLU	GLY	TYR	GLN	TYR
2	ARG	ALA	LEU	TYR	ASP	TYR	LYS	LYS	GLU	ARG
3	GLU	GLU	ASP	ILE	ASP	LEU	HIS	LEU	GLY	ASP
4	ILE	LEU	THR	VAL	ASN	LYS	GLY	SER	LEU	VAL
5	ALA	LEU	GLY	PHE	SER	ASP	GLY	GLN	GLU	ALA
6	ARG	PRO	GLU	GLU	ILE	GLY	TRP	LEU	ASN	GLY
7	TYR	ASN	GLU	THR	THR	GLY	GLU	ARG	GLY	ASP
8	PHE	PRO	GLY	THR	TYR	VAL	GLU	TYR	ILE	GLY
9	ARG	LYS	LYS	ILE	SER	PRO	TRP	ASN	SER	SER