BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17126

Title: NMR assigment of Heparanase construct Hep158-417

Deposition date: 2010-08-13 Original release date: 2010-09-02

Authors: Mosulen Machuca, Silvia; Orti, Leticia; Bas, Esperanza; Carbajo, Rodrigo; Pineda-Lucena, Antonio

Citation: Mosulen Machuca, Silvia; Orti, Leticia; Bas, Esperanza; Carbajo, Rodrigo; Pineda-Lucena, Antonio. "Assigned Chemical Shifts for Heparanase 158-417"  .

Assembly members:
HEP158-417, polymer, 282 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: p11

Entity Sequences (FASTA):
HEP158-417: GSSHHHHHHSSGRENLYFQG HMKKFKNSTYSRSSVDVLYT FANCSGLDLIFGLNALLRTA DLQWNSSNAQLLLDYCSSKG YNISWELGNEPNSFLKKADI FINGSQLGEDFIQLHKLLRK STFKNAKLYGPDVGQPRRKT AKMLKSFLKAGGEVIDSVTW HHYYLNGRTATREDFLNPDV LDIFISSVQKVFQVVESTRP GKKVWLGETSSAYGGGAPLL SDTFAAGFMWLDKLGLSARM GIEVVMRQVFFGAGNYHLVD ENFDPLPDYWLSLLFKKLVG TK

Data sets:
Data typeCount
13C chemical shifts808
15N chemical shifts258
1H chemical shifts1645

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11

Entities:

Entity 1, subunit 1 282 residues - Formula weight is not available

Residues at the N-term GSSHHHHHHSSGRENLYFQGHM correspond to an affinity tag and their chemical shifts are not reported

1   GLYSERSERHISHISHISHISHISHISSER
2   SERGLYARGGLUASNLEUTYRPHEGLNGLY
3   HISMETLYSLYSPHELYSASNSERTHRTYR
4   SERARGSERSERVALASPVALLEUTYRTHR
5   PHEALAASNCYSSERGLYLEUASPLEUILE
6   PHEGLYLEUASNALALEULEUARGTHRALA
7   ASPLEUGLNTRPASNSERSERASNALAGLN
8   LEULEULEUASPTYRCYSSERSERLYSGLY
9   TYRASNILESERTRPGLULEUGLYASNGLU
10   PROASNSERPHELEULYSLYSALAASPILE
11   PHEILEASNGLYSERGLNLEUGLYGLUASP
12   PHEILEGLNLEUHISLYSLEULEUARGLYS
13   SERTHRPHELYSASNALALYSLEUTYRGLY
14   PROASPVALGLYGLNPROARGARGLYSTHR
15   ALALYSMETLEULYSSERPHELEULYSALA
16   GLYGLYGLUVALILEASPSERVALTHRTRP
17   HISHISTYRTYRLEUASNGLYARGTHRALA
18   THRARGGLUASPPHELEUASNPROASPVAL
19   LEUASPILEPHEILESERSERVALGLNLYS
20   VALPHEGLNVALVALGLUSERTHRARGPRO
21   GLYLYSLYSVALTRPLEUGLYGLUTHRSER
22   SERALATYRGLYGLYGLYALAPROLEULEU
23   SERASPTHRPHEALAALAGLYPHEMETTRP
24   LEUASPLYSLEUGLYLEUSERALAARGMET
25   GLYILEGLUVALVALMETARGGLNVALPHE
26   PHEGLYALAGLYASNTYRHISLEUVALASP
27   GLUASNPHEASPPROLEUPROASPTYRTRP
28   LEUSERLEULEUPHELYSLYSLEUVALGLY
29   THRLYS

Samples:

sample_1: HEP158-417, [U-100% 15N], 900 uM; sodium chloride 50 mM; sodium phosphate 25 mM; N-Lauroyl-sarcosine 0.125%; H2O 95%; D2O 5%

sample_2: HEP158-417, [U-99% 13C; U-99% 15N], 900 uM; sodium chloride 50 mM; sodium phosphate 25 mM; N-Lauroyl-sarcosine 0.125%; H2O 95%; D2O 5%

sample_3: HEP158-417, [U-100% 13C; U-100% 15N; U-80% 2H], 900 uM; sodium chloride 50 mM; sodium phosphate 25 mM; N-Lauroyl-sarcosine 0.125%; H2O 95%; D2O 5%

sample_4: HEP158-417 900 uM; sodium chloride 50 mM; sodium phosphate 25 mM; N-Lauroyl-sarcosine 0.125%; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_4isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_4isotropicsample_conditions_1
3D HNHAsample_2isotropicsample_conditions_1
3D HNHBsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_4isotropicsample_conditions_1
3D HCACOsample_3isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - processing

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 900 MHz

Related Database Links:

GB AAD41342 AAD45379 AAD45669 AAD54516 AAD54941
REF XP_007997297 XP_011765811

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts