BMRB Entry 17137
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17137
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Title: Rhodanese Y PubMed: 20960078
Deposition date: 2010-08-20 Original release date: 2010-10-26
Authors: Li, Hongwei; Jin, Changwen
Citation: Li, Hongwei; Bi, Yunchen; Xia, Bin; Jin, Changwen. "(1)H, (13)C and (15)N resonance assignments of the rhodanese domain of YgaP from Escherichia coli." Biomol. NMR Assignments 5, 101-103 (2011).
Assembly members:
YgaP, polymer, 107 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28
Entity Sequences (FASTA):
YgaP: MALTTISPHDAQELIARGAK
LIDIRDADEYLREHIPEADL
APLSVLEQSGLPAKLRHEQI
IFHCQAGKRTSNNADKLAAI
AAPAEIFLLEDGIDGWKRAG
LPVAVNK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 451 |
15N chemical shifts | 108 |
1H chemical shifts | 751 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | YgaP | 1 |
Entities:
Entity 1, YgaP 107 residues - Formula weight is not available
1 | MET | ALA | LEU | THR | THR | ILE | SER | PRO | HIS | ASP | ||||
2 | ALA | GLN | GLU | LEU | ILE | ALA | ARG | GLY | ALA | LYS | ||||
3 | LEU | ILE | ASP | ILE | ARG | ASP | ALA | ASP | GLU | TYR | ||||
4 | LEU | ARG | GLU | HIS | ILE | PRO | GLU | ALA | ASP | LEU | ||||
5 | ALA | PRO | LEU | SER | VAL | LEU | GLU | GLN | SER | GLY | ||||
6 | LEU | PRO | ALA | LYS | LEU | ARG | HIS | GLU | GLN | ILE | ||||
7 | ILE | PHE | HIS | CYS | GLN | ALA | GLY | LYS | ARG | THR | ||||
8 | SER | ASN | ASN | ALA | ASP | LYS | LEU | ALA | ALA | ILE | ||||
9 | ALA | ALA | PRO | ALA | GLU | ILE | PHE | LEU | LEU | GLU | ||||
10 | ASP | GLY | ILE | ASP | GLY | TRP | LYS | ARG | ALA | GLY | ||||
11 | LEU | PRO | VAL | ALA | VAL | ASN | LYS |
Samples:
sample_1: YgaP 1 mM; TRIS 20 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.02 M; pH: 7.2; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
BMRB | 19943 19946 25085 |
PDB | |
DBJ | BAB36952 BAE76780 BAG78445 BAI26930 BAI31960 |
EMBL | CAQ33005 CAQ87965 CAQ99590 CAR04179 CAR09287 |
GB | AAC75715 AAG57776 AAN44189 AAN81669 AAP18017 |
REF | NP_311556 NP_417154 NP_708482 WP_001229433 WP_001229437 |
SP | P55734 |
AlphaFold | P55734 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts