BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17137

Title: Rhodanese Y   PubMed: 20960078

Deposition date: 2010-08-20 Original release date: 2010-10-26

Authors: Li, Hongwei; Jin, Changwen

Citation: Li, Hongwei; Bi, Yunchen; Xia, Bin; Jin, Changwen. "(1)H, (13)C and (15)N resonance assignments of the rhodanese domain of YgaP from Escherichia coli."  Biomol. NMR Assignments 5, 101-103 (2011).

Assembly members:
YgaP, polymer, 107 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):
YgaP: MALTTISPHDAQELIARGAK LIDIRDADEYLREHIPEADL APLSVLEQSGLPAKLRHEQI IFHCQAGKRTSNNADKLAAI AAPAEIFLLEDGIDGWKRAG LPVAVNK

Data sets:
Data typeCount
13C chemical shifts451
15N chemical shifts108
1H chemical shifts751

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YgaP1

Entities:

Entity 1, YgaP 107 residues - Formula weight is not available

1   METALALEUTHRTHRILESERPROHISASP
2   ALAGLNGLULEUILEALAARGGLYALALYS
3   LEUILEASPILEARGASPALAASPGLUTYR
4   LEUARGGLUHISILEPROGLUALAASPLEU
5   ALAPROLEUSERVALLEUGLUGLNSERGLY
6   LEUPROALALYSLEUARGHISGLUGLNILE
7   ILEPHEHISCYSGLNALAGLYLYSARGTHR
8   SERASNASNALAASPLYSLEUALAALAILE
9   ALAALAPROALAGLUILEPHELEULEUGLU
10   ASPGLYILEASPGLYTRPLYSARGALAGLY
11   LEUPROVALALAVALASNLYS

Samples:

sample_1: YgaP 1 mM; TRIS 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.02 M; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 19943 19946 25085
PDB
DBJ BAB36952 BAE76780 BAG78445 BAI26930 BAI31960
EMBL CAQ33005 CAQ87965 CAQ99590 CAR04179 CAR09287
GB AAC75715 AAG57776 AAN44189 AAN81669 AAP18017
REF NP_311556 NP_417154 NP_708482 WP_001229433 WP_001229437
SP P55734
AlphaFold P55734

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts