BMRB Entry 17148

Title:
N-terminal domain of Nephila clavipes major ampulate spidroin 1
Deposition date:
2010-08-25
Original release date:
2010-09-13
Authors:
Parnham, Stuart; Hennig, Mirko
Citation:

Citation: Parnham, Stuart; Gaines, William; Duggan, Brendan; Marcotte, William; Hennig, Mirko. "NMR assignments of the N-terminal domain of Nephila clavipes spidroin 1."  Biomol. NMR Assignments 5, 131-133 (2011).
PubMed: 21152998

Assembly members:

Assembly members:
Spn-1, polymer, 144 residues, 14961.5253 Da.

Natural source:

Natural source:   Common Name: Nephila clavipes   Taxonomy ID: 6915   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Nephila clavipes

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: GinG

Data typeCount
13C chemical shifts539
15N chemical shifts160
1H chemical shifts872
coupling constants98

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Spn-11

Entities:

Entity 1, Spn-1 144 residues - 14961.5253 Da.

1   GLYPROLEUGLYSERPROGLYILEPROGLY
2   GLNASNTHRPROTRPSERSERTHRGLULEU
3   ALAASPALAPHEILEASNALAPHEMETASN
4   GLUALAGLYARGTHRGLYALAPHETHRALA
5   ASPGLNLEUASPASPMETSERTHRILEGLY
6   ASPTHRILELYSTHRALAMETASPLYSMET
7   ALAARGSERASNLYSSERSERLYSGLYLYS
8   LEUGLNALALEUASNMETALAPHEALASER
9   SERMETALAGLUILEALAALAVALGLUGLN
10   GLYGLYLEUSERVALASPALALYSTHRASN
11   ALAILEALAASPSERLEUASNSERALAPHE
12   TYRGLNTHRTHRGLYALAALAASNPROGLN
13   PHEVALASNGLUILEARGSERLEUILEASN
14   METPHEALAGLNSERSERALAASNGLUVAL
15   SERTYRGLYGLY

Samples:

Spn-1: Spn-1, [U-98% 13C; U-98% 15N], 1.0 mM; H2O 1.0 mM; D2O 1.0 mM

sample_conditions_1: ionic strength: 500 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
aro_d20noesy (H[c]_H[c].NOESY)Spn-1isotropicsample_conditions_1
3D C(CO)NHSpn-1isotropicsample_conditions_1
2D 1H-15N HSQC/HMQCSpn-1isotropicsample_conditions_1
cbca_500mM (H[N[co[{CA|ca[C]}]]])Spn-1isotropicsample_conditions_1
3D 1H-15N NOESYSpn-1isotropicsample_conditions_1
3D 1H-13C NOESYSpn-1isotropicsample_conditions_1
3D H(CCO)NHSpn-1isotropicsample_conditions_1
500mM_aro (h[C]_H.TOCSY)Spn-1isotropicsample_conditions_1
HCaN_prolines (HCA[N])Spn-1isotropicsample_conditions_1
hnco_500mM (HNCO)Spn-1isotropicsample_conditions_1
hnha (H{[N]+[HA]})Spn-1isotropicsample_conditions_1
hbhaconh_500 (H{ca|cca}[co[N]]HA)Spn-1isotropicsample_conditions_1

Software:

NMRDraw vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum analysis, Spectrum display

NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum processing

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

ANALYSIS v2.0, CCPN - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

GB ACF19411

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks