BMRB Entry 17179
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17179
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Title: NMR Studies of the Backbone Flexibility and Structure of Human Growth Hormone: A Comparison of High and Low pH Conformations PubMed: 12054815
Deposition date: 2010-09-10 Original release date: 2010-11-10
Authors: Kasimova, Marina; Kristensen, Soren; Howe, Peter; Christensen, Thorkild; Matthiesen, Finn; Petersen, Jorgen; Sorensen, Hans; Led, Jens
Citation: Kasimova, Marina; Kristensen, Soren; Howe, Peter; Christensen, Thorkild; Matthiesen, Finn; Petersen, Jorgen; Sorensen, Hans; Led, Jens. "NMR Studies of the Backbone Flexibility and Structure of Human Growh Hormone: A Comparison of High and Low pH Conformations" J. Mol. Biol. 318, 679-695 (2002).
Assembly members:
rhGH, polymer, 191 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
rhGH: FPTIPLSRLFDNAMLRAHRL
HQLAFDTYEEFEEAYIPKEQ
KYSFLQNPQASLCFSESIPT
PSNREQAQQKSNLQLLRISL
LLIQSWLEPVGFLRSVFANS
LVYGASDSDVYDLLKDLEEG
IQTLMGRLEDGSPRTGQIFK
QTYSKFDANSHNDDALLKNY
GLLYCFRKDMDKVETFLRIV
QCRSVEGSCGF
- chemical_rates
| Data type | Count |
| kinetic rates | 182 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | rhGH | 1 |
Entities:
Entity 1, rhGH 191 residues - Formula weight is not available
| 1 | PHE | PRO | THR | ILE | PRO | LEU | SER | ARG | LEU | PHE | ||||
| 2 | ASP | ASN | ALA | MET | LEU | ARG | ALA | HIS | ARG | LEU | ||||
| 3 | HIS | GLN | LEU | ALA | PHE | ASP | THR | TYR | GLU | GLU | ||||
| 4 | PHE | GLU | GLU | ALA | TYR | ILE | PRO | LYS | GLU | GLN | ||||
| 5 | LYS | TYR | SER | PHE | LEU | GLN | ASN | PRO | GLN | ALA | ||||
| 6 | SER | LEU | CYS | PHE | SER | GLU | SER | ILE | PRO | THR | ||||
| 7 | PRO | SER | ASN | ARG | GLU | GLN | ALA | GLN | GLN | LYS | ||||
| 8 | SER | ASN | LEU | GLN | LEU | LEU | ARG | ILE | SER | LEU | ||||
| 9 | LEU | LEU | ILE | GLN | SER | TRP | LEU | GLU | PRO | VAL | ||||
| 10 | GLY | PHE | LEU | ARG | SER | VAL | PHE | ALA | ASN | SER | ||||
| 11 | LEU | VAL | TYR | GLY | ALA | SER | ASP | SER | ASP | VAL | ||||
| 12 | TYR | ASP | LEU | LEU | LYS | ASP | LEU | GLU | GLU | GLY | ||||
| 13 | ILE | GLN | THR | LEU | MET | GLY | ARG | LEU | GLU | ASP | ||||
| 14 | GLY | SER | PRO | ARG | THR | GLY | GLN | ILE | PHE | LYS | ||||
| 15 | GLN | THR | TYR | SER | LYS | PHE | ASP | ALA | ASN | SER | ||||
| 16 | HIS | ASN | ASP | ASP | ALA | LEU | LEU | LYS | ASN | TYR | ||||
| 17 | GLY | LEU | LEU | TYR | CYS | PHE | ARG | LYS | ASP | MET | ||||
| 18 | ASP | LYS | VAL | GLU | THR | PHE | LEU | ARG | ILE | VAL | ||||
| 19 | GLN | CYS | ARG | SER | VAL | GLU | GLY | SER | CYS | GLY | ||||
| 20 | PHE |
Samples:
sample_1: rhGH0.7 – 1 mM; H2O 99.996%
sample_conditions_1: pH: 7.0; pressure: ambient atm; temperature: 305 K
sample_conditions_2: pH: 2.7; pressure: ambient atm; temperature: 305 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HN exchange | sample_1 | isotropic | sample_conditions_2 |
| HN exchange | sample_1 | isotropic | sample_conditions_1 |
Software:
software, na - na
NMR spectrometers:
- Varian Unity Inova 500 MHz
Related Database Links:
| PDB |