BMRB Entry 17181
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17181
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Citation: Kitahara, Ryo; Yokoyama, Shigeyuki; Akasaka, Kazuyuki. "NMR Snapshots of a Fluctuating Protein Structure: Ubiquitin at 30 bar-3 kbar" J. Mol. Biol. 347, 277-285 (2005).
PubMed: 15740740
Assembly members:
ubiquitin, polymer, 76 residues, Formula weight is not available
Natural source: Common Name: cattle Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
ubiquitin: MQIFVKTLTGKTITLEVEPS
DTIENVKAKIQDKEGIPPDQ
QRLIFAGKQLEDGRTLSDYN
IQKESTLHLVLRLRGG
- chemical_rates
| Data type | Count |
| kinetic rates | 4 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ubiquitin | 1 |
Entities:
Entity 1, ubiquitin 76 residues - Formula weight is not available
| 1 | MET | GLN | ILE | PHE | VAL | LYS | THR | LEU | THR | GLY | ||||
| 2 | LYS | THR | ILE | THR | LEU | GLU | VAL | GLU | PRO | SER | ||||
| 3 | ASP | THR | ILE | GLU | ASN | VAL | LYS | ALA | LYS | ILE | ||||
| 4 | GLN | ASP | LYS | GLU | GLY | ILE | PRO | PRO | ASP | GLN | ||||
| 5 | GLN | ARG | LEU | ILE | PHE | ALA | GLY | LYS | GLN | LEU | ||||
| 6 | GLU | ASP | GLY | ARG | THR | LEU | SER | ASP | TYR | ASN | ||||
| 7 | ILE | GLN | LYS | GLU | SER | THR | LEU | HIS | LEU | VAL | ||||
| 8 | LEU | ARG | LEU | ARG | GLY | GLY |
Samples:
sample_1: deuterated acetate buffer 30 mM; ubiquitin, [U-100% 15N], 3.0 mM; H2O 95%; D2O 95%
sample_conditions_1: pH: 4.6; pressure: 29.61 atm; temperature: 293 K
sample_conditions_2: pH: 4.6; pressure: 2960 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NH-NOE | sample_1 | isotropic | sample_conditions_1 |
| NH-NOE | sample_1 | isotropic | sample_conditions_2 |
Software:
ModelFree v4.15, Palmer - data analysis
CYANA, Guntert, Mumenthaler and Wuthrich - data analysis
NMR spectrometers:
- Bruker DRX 800 MHz
Related Database Links:
| BMRB | 11505 11547 15047 15410 15689 15907 16228 16582 16626 16895 17439 17769 17919 18582 18583 18584 18610 18611 18737 19406 19412 25070 25123 25601 26604 4245 4375 |
| PDB | |
| DBJ | BAA03983 BAA09860 BAA11842 BAA11843 BAA23486 |
| EMBL | CAA25706 CAA26488 CAA28495 CAA30183 CAA30815 |
| GB | AAA02769 AAA28154 AAA28997 AAA28998 AAA28999 |
| PIR | I50437 I51568 I65237 JN0790 S13928 |
| PRF | 0412265A 1101405A 1212243A 1212243B 1212243C |
| REF | NP_001005123 NP_001006688 NP_001009117 NP_001009202 NP_001009286 |
| SP | P0C273 P0C275 P0C276 P0CG47 P0CG48 |
| TPD | FAA00319 |
| TPE | CEL68433 CEL70397 CEL75964 CEL78064 |
| TPG | DAA18802 DAA20663 DAA20672 DAA24675 DAA28295 |
| AlphaFold | P0C273 P0C275 P0C276 P0CG47 P0CG48 |