BMRB Entry 17184
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17184
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Title: Lactose Binding to Galectin-1 Modulates Structural Dynamics, Increases Conformational Entropy, and Occurs with Apparent Negative Cooperativity PubMed: 20184898
Deposition date: 2010-09-10 Original release date: 2010-11-10
Authors: Nesmelova, Irina; Ermakova, Elena; Daragan, Vladimir; Pang, Mabel; Menendez, Margarita; Lagartera, Laura; Solis, Dolores; Baum, Linda; Mayo, Kevin
Citation: Nesmelova, Irina; Ermakova, Elena; Daragan, Vladimir; Pang, Mabel; Menendez, Margarita; Lagartera, Laura; Solis, Dolores; Baum, Linda; Mayo, Kevin. "Lactose Binding to Galectin-1 Modulates Structural Dynamics, Increases Conformational Entropy, and Occurs with Apparent Negative Cooperativity" J. Mol. Biol. 397, 1209-1230 (2010).
Assembly members:
gal1, polymer, 135 residues, Formula weight is not available
LAT, SACCHARIDE, 342.296 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
gal1: MACGLVASNLNLKPGECLRV
RGEVAPDAKSFVLNLGKDSN
NLCLHFNPRFNAHGDANTIV
CNSKDGGAWGTEQREAVFPF
QPGSVAEVCITFDQANLTVK
LPDGYEFKFPNRLNLEAINY
MAADGDFKIKCVAFD
- binding constants
- pKa values
| Data type | Count |
| binding constants | 2 |
| pKa values | 1 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | gal1 | 1 |
| 2 | LAT | 2 |
Entities:
Entity 1, gal1 135 residues - Formula weight is not available
| 1 | MET | ALA | CYS | GLY | LEU | VAL | ALA | SER | ASN | LEU | ||||
| 2 | ASN | LEU | LYS | PRO | GLY | GLU | CYS | LEU | ARG | VAL | ||||
| 3 | ARG | GLY | GLU | VAL | ALA | PRO | ASP | ALA | LYS | SER | ||||
| 4 | PHE | VAL | LEU | ASN | LEU | GLY | LYS | ASP | SER | ASN | ||||
| 5 | ASN | LEU | CYS | LEU | HIS | PHE | ASN | PRO | ARG | PHE | ||||
| 6 | ASN | ALA | HIS | GLY | ASP | ALA | ASN | THR | ILE | VAL | ||||
| 7 | CYS | ASN | SER | LYS | ASP | GLY | GLY | ALA | TRP | GLY | ||||
| 8 | THR | GLU | GLN | ARG | GLU | ALA | VAL | PHE | PRO | PHE | ||||
| 9 | GLN | PRO | GLY | SER | VAL | ALA | GLU | VAL | CYS | ILE | ||||
| 10 | THR | PHE | ASP | GLN | ALA | ASN | LEU | THR | VAL | LYS | ||||
| 11 | LEU | PRO | ASP | GLY | TYR | GLU | PHE | LYS | PHE | PRO | ||||
| 12 | ASN | ARG | LEU | ASN | LEU | GLU | ALA | ILE | ASN | TYR | ||||
| 13 | MET | ALA | ALA | ASP | GLY | ASP | PHE | LYS | ILE | LYS | ||||
| 14 | CYS | VAL | ALA | PHE | ASP |
Entity 2, LAT - 342.296 Da.
| 1 | LAT |
Samples:
sample_1: gal1, [U-13C; U-15N], 34 – 2000 uM; potassium phosphate buffer 20 mM; deuterated DTT 2 mM; LAT0 – 100 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 7; pressure: ambient atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - visualization
NMR spectrometers:
- Varian Unity Inova 800 MHz