BMRB Entry 17187

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17187

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Title:
Elucidation of the poly-L-proline binding site in Acanthamoeba profilin I by NMR spectroscopy
Deposition date:
2010-09-10
Original release date:
2010-11-10
Authors:
Archer, Sharon; Vinson, Valda; Pollard, Thomas; Torchia, Dennis
Citation:

Citation: Archer, Sharon; Vinson, Valda; Pollard, Thomas; Torchia, Dennis. "Elucidation of the poly-L-proline binding site in Acanthamoeba profilin I by NMR spectroscopy"  FEBS Lett. 337, 145-151 (1994).
PubMed: 8287969

Assembly members:

Assembly members:
profilinI, polymer, 126 residues, Formula weight is not available
pLp, polymer, 10 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Acanthamoeba castellanii   Taxonomy ID: 5755   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Acanthamoeba castellanii

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
profilinI: MSWQTYVDTNLVGTGAVTQA AILGLDGNTWATSAGFAVTP AQGQTLASAFNNADPIRASG FDLAGVHYVTLRADDRSIYG KKGSAGVITVKTSKSILVGV YNEKIQPGTAANVVEKLADY LIGQGF
pLp: PPPPPPPPPP

Data sets:
  • binding constants
Data typeCount
binding constants8

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1profilinI1
2pLp2

Entities:

Entity 1, profilinI 126 residues - Formula weight is not available

1   METSERTRPGLNTHRTYRVALASPTHRASN
2   LEUVALGLYTHRGLYALAVALTHRGLNALA
3   ALAILELEUGLYLEUASPGLYASNTHRTRP
4   ALATHRSERALAGLYPHEALAVALTHRPRO
5   ALAGLNGLYGLNTHRLEUALASERALAPHE
6   ASNASNALAASPPROILEARGALASERGLY
7   PHEASPLEUALAGLYVALHISTYRVALTHR
8   LEUARGALAASPASPARGSERILETYRGLY
9   LYSLYSGLYSERALAGLYVALILETHRVAL
10   LYSTHRSERLYSSERILELEUVALGLYVAL
11   TYRASNGLULYSILEGLNPROGLYTHRALA
12   ALAASNVALVALGLULYSLEUALAASPTYR
13   LEUILEGLYGLNGLYPHE

Entity 2, pLp 10 residues - Formula weight is not available

1   PROPROPROPROPROPROPROPROPROPRO

Related Database Links:

PDB 1PRQ 2PRF 1AWI
GB AAA27710 ELR22869
REF XP_004351646
SP P68696
AlphaFold P68696