BMRB Entry 17195
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17195
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone assignments for the UBA Domain of E2-25K PubMed: 21281599
Deposition date: 2010-09-15 Original release date: 2011-03-24
Authors: Wilson, Randall; Edmondson, Stephen; Flatt, Justin; Twigg, Pamela
Citation: Wilson, Randall; Edmondson, Stephen; Flatt, Justin; Helms, Kimberli; Twigg, Pamela. "The E2-25K ubiquitin-associated (UBA) domain aids in polyubiquitin chain synthesis and linkage specificity" Biochem. Biophys. Res. Commun. 405, 662-666 (2011).
Assembly members:
E2-25K_UBA_domain, polymer, 70 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b
Entity Sequences (FASTA):
E2-25K_UBA_domain: MGSSHHHHHHSSGLVPEGSH
MASGAPVSSPEYTKKIENLC
AMGFDRNAVIVALSSKSWDV
ETATELLLSN
- assigned_chemical_shifts
- RDCs
Data type | Count |
13C chemical shifts | 155 |
15N chemical shifts | 50 |
1H chemical shifts | 50 |
residual dipolar couplings | 37 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | UBA monomer | 1 |
Entities:
Entity 1, UBA monomer 70 residues - Formula weight is not available
Residues 1-23 represent a non-native affinity tag and residues 24-70 represent residues 154-200 of E2-25K
1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | |
2 | SER | SER | GLY | LEU | VAL | PRO | GLU | GLY | SER | HIS | |
3 | MET | ALA | SER | GLY | ALA | PRO | VAL | SER | SER | PRO | |
4 | GLU | TYR | THR | LYS | LYS | ILE | GLU | ASN | LEU | CYS | |
5 | ALA | MET | GLY | PHE | ASP | ARG | ASN | ALA | VAL | ILE | |
6 | VAL | ALA | LEU | SER | SER | LYS | SER | TRP | ASP | VAL | |
7 | GLU | THR | ALA | THR | GLU | LEU | LEU | LEU | SER | ASN |
Samples:
sample_1: E2-25K UBA domain, [U-100% 13C; U-100% 15N], 1.4 mM; DSS 0.12 mM; sodium azide 0.9 % v/v; sodium phosphate 50 mM; sodium chloride 100 mM; beta-mercaptoethanol 0.1 mM; H2O 90%; D2O 10%
RDC_sample: E2-25K UBA domain, [U-100% 15N], 0.98 mM; DSS 0.12 mM; sodium phosphate 50 mM; sodium chloride 100 mM; beta-mercaptoethanol 0.1 mM; n-dodecyl hexa(ethylene glycol) 5%; n-hexanol 5%; H2O 90%; D2O 10%
sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 273 K
RDC_conditions: pH: 7; pressure: 1 atm; temperature: 273 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
2D Nhsqc-ipap | RDC_sample | isotropic | RDC_conditions |
2D NHSC S3 | RDC_sample | isotropic | RDC_conditions |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking
VNMRJ, Varian - collection
Module, Dosset, P., Hus, J. C., Marion, D., and Blackledge, M - data analysis
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts