BMRB Entry 17201

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17201

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Title:
Backbone assignment of the little finger domain of Y-family DNA polymerase Dpo4
Deposition date:
2010-09-22
Original release date:
2011-08-03
Authors:
Ma, Dejian; Fowler, Jason
Citation:

Citation: Ma, Dejian; Fowler, Jason; Suo, Zucai. "Backbone assignment of the little finger domain of a Y-family DNA polymerase."  Biomol. NMR Assignments 5, 195-198 (2011).
PubMed: 21337030

Assembly members:

Assembly members:
Dpo4, polymer, 131 residues, 27957.5 Da.

Natural source:

Natural source:   Common Name: Sulfolobus solfataricus   Taxonomy ID: 2287   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus solfataricus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Dpo4: MDEYNEPIRTRVRKSIGRIV TMKRNSRNLEEIKPYLFRAI EESYYKLDKRIPKAIHVVAV TEDLDIVSRGRTFPHGISKE TAYSESVKLLQKILEEDERK IRRIGVRFSKFIEAIGLDKF FDTLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts200
15N chemical shifts118
1H chemical shifts118

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1little finger domain of Dpo41

Entities:

Entity 1, little finger domain of Dpo4 131 residues - 27957.5 Da.

Last 8 residues represent a non-native affinity tag.

1   METASPGLUTYRASNGLUPROILEARGTHR
2   ARGVALARGLYSSERILEGLYARGILEVAL
3   THRMETLYSARGASNSERARGASNLEUGLU
4   GLUILELYSPROTYRLEUPHEARGALAILE
5   GLUGLUSERTYRTYRLYSLEUASPLYSARG
6   ILEPROLYSALAILEHISVALVALALAVAL
7   THRGLUASPLEUASPILEVALSERARGGLY
8   ARGTHRPHEPROHISGLYILESERLYSGLU
9   THRALATYRSERGLUSERVALLYSLEULEU
10   GLNLYSILELEUGLUGLUASPGLUARGLYS
11   ILEARGARGILEGLYVALARGPHESERLYS
12   PHEILEGLUALAILEGLYLEUASPLYSPHE
13   PHEASPTHRLEUGLUHISHISHISHISHIS
14   HIS

Related Database Links:

SWS Q97W02
PDB 1JX4 1JXL 1N48 1N56 1RYR 1RYS 1S0M 1S0N 1S0O 1S10 1S97 1S9F 2AGO 2AGP 2AGQ 2ASD 2ASJ 2ASL 2ATL 2AU0 2BQ3 2BQR 2BQU 2BR0 2C22 2C28 2C2D 2C2E 2C2R 2IA6 2IBK 2IMW 2J6S 2J6T 2J6U 2JEF 2JEG 2JEI 2JEJ 2R8G 2R8H 2R8I 2RDI 2RDJ 2UVR 2UVU 2UVV 2UVW 2V4Q 2V4R 2V9W 2VA2 2VA3 2W8K 2W8L 2W9A 2W9B 2W9C 2XC9 2XCA 2XCP 3FDS 3GII 3GIJ 3GIK 3GIL 3GIM 3KHG 3KHH 3KHL 3KHR 3M9M 3M9N 3M9O 3PR4 3PR5 3PVX 3PW0 3PW2 3PW4 3PW5 3PW7 3QZ7 3QZ8 3RAQ 3RAX 3RB0 3RB3 3RB4 3RB6 3RBD 3RBE 3T5H 3T5J 3T5K 3T5L 3V6H 3V6J 3V6K 4F4W 4F4X 4FBT 4FBU 4G3I 4GC6 4GC7 4JUZ 4JV0 4JV1 4JV2 4QWB 4QWC 4RUA 4RUC 4RZR 4TQR 4TQS
GB AAK42588 ABA03150 ACX90547 AKA72680 AKA75380
REF WP_009993137
SP Q97W02
AlphaFold Q97W02

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks