BMRB Entry 17201

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17201

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Title:
Backbone assignment of the little finger domain of Y-family DNA polymerase Dpo4
Deposition date:
2010-09-22
Original release date:
2011-08-03
Authors:
Ma, Dejian; Fowler, Jason
Citation:

Citation: Ma, Dejian; Fowler, Jason; Suo, Zucai. "Backbone assignment of the little finger domain of a Y-family DNA polymerase."  Biomol. NMR Assignments 5, 195-198 (2011).
PubMed: 21337030

Assembly members:

Assembly members:
Dpo4, polymer, 131 residues, 27957.5 Da.

Natural source:

Natural source:   Common Name: Sulfolobus solfataricus   Taxonomy ID: 2287   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus solfataricus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Dpo4: MDEYNEPIRTRVRKSIGRIV TMKRNSRNLEEIKPYLFRAI EESYYKLDKRIPKAIHVVAV TEDLDIVSRGRTFPHGISKE TAYSESVKLLQKILEEDERK IRRIGVRFSKFIEAIGLDKF FDTLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts200
15N chemical shifts118
1H chemical shifts118

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1little finger domain of Dpo41

Entities:

Entity 1, little finger domain of Dpo4 131 residues - 27957.5 Da.

Last 8 residues represent a non-native affinity tag.

1   METASPGLUTYRASNGLUPROILEARGTHR
2   ARGVALARGLYSSERILEGLYARGILEVAL
3   THRMETLYSARGASNSERARGASNLEUGLU
4   GLUILELYSPROTYRLEUPHEARGALAILE
5   GLUGLUSERTYRTYRLYSLEUASPLYSARG
6   ILEPROLYSALAILEHISVALVALALAVAL
7   THRGLUASPLEUASPILEVALSERARGGLY
8   ARGTHRPHEPROHISGLYILESERLYSGLU
9   THRALATYRSERGLUSERVALLYSLEULEU
10   GLNLYSILELEUGLUGLUASPGLUARGLYS
11   ILEARGARGILEGLYVALARGPHESERLYS
12   PHEILEGLUALAILEGLYLEUASPLYSPHE
13   PHEASPTHRLEUGLUHISHISHISHISHIS
14   HIS

Samples:

sample_1: Dpo4, [U-100% 13C; U-100% 15N], 0.4 mM; H2O 95%; D2O 5%; NaCl80 – 200 mM; phosphate buffer 20 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 7.0; pressure: 1 atm; temperature: 323 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking, processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

MARS, Jung and Zweckstetter - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

SWS Q97W02
PDB
GB AAK42588 ABA03150 ACX90547 AKA72680 AKA75380
REF WP_009993137
SP Q97W02
AlphaFold Q97W02

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks