BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17224

Title: TROSY-NMR reveals interaction between ERp57 and the tip of the calreticulin P-domain   PubMed: 11842220

Deposition date: 2010-09-29 Original release date: 2010-11-10

Authors: Frickel, Eva-Maria; Riek, Roland; Jelesarov, Ilian; Helenius, Ari; Wuthrich, Kurt; Ellgaard, Lars

Citation: Frickel, Eva-Maria; Riek, Roland; Jelesarov, Ilian; Helenius, Ari; Wuthrich, Kurt; Ellgaard, Lars. "TROSY-NMR reveals interaction between ERp57 and the tip of the calreticulin P-domain"  Proc. Natl. Acad. Sci. U.S.A. 99, 1954-1959 (2002).

Assembly members:
CRT, polymer, 100 residues, Formula weight is not available
ERp57, polymer, 505 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metaoza   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHisERp57

Entity Sequences (FASTA):
CRT: SQVESGSLEDDWDFLPPKKI KDPDASKPEDWDERAKIDDP TDSKPEDWDKPEHIPDPDAK KPEDWDEEMDGEWEPPVIQN PEYKGEWKPRQIDNPDYKGT
ERp57: MRLRRLALFPGVALLLAAAR LAAASDVLELTDDNFESRIS DTGSAGLMLVEFFAPWCGHC KRLAPEYEAAATRLKGIVPL AKVDCTANTNTCNKYGVSGY PTLKIFRDGEEAGAYDGPRT ADGIVSHLKKQAGPASVPLR TEEEFKKFISDKDASIVGFF DDSFSEAHSEFLKAASNLRD NYRFAHTNVESLVNEYDDNG EGIILFRPSHLTNKFEDKTV AYTEQKMTSGKIKKFIQENI FGICPHMTEDNKDLIQGKDL LIAYYDVDYEKNAKGSNYWR NRVMMVAKKFLDAGHKLNFA VASRKTFSHELSDFGLESTA GEIPVVAIRTAKGEKFVMQE EFSRDGKALERFLQDYFDGN LKRYLKSEPIPESNDGPVKV VVAENFDEIVNNENKDVLIE FYAPWCGHCKNLEPKYKELG EKLSKDPNIVIAKMDATAND VPSPYEVRGFPTIYFSPANK KLNPKKYEGGRELSDFISYL QREATNPPVIQEEKPKKKKK AQEDL

Data sets:
Data typeCount
binding constants7
kinetic rates1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CRT1
2ERp572

Entities:

Entity 1, CRT 100 residues - Formula weight is not available

1   SERGLNVALGLUSERGLYSERLEUGLUASP
2   ASPTRPASPPHELEUPROPROLYSLYSILE
3   LYSASPPROASPALASERLYSPROGLUASP
4   TRPASPGLUARGALALYSILEASPASPPRO
5   THRASPSERLYSPROGLUASPTRPASPLYS
6   PROGLUHISILEPROASPPROASPALALYS
7   LYSPROGLUASPTRPASPGLUGLUMETASP
8   GLYGLUTRPGLUPROPROVALILEGLNASN
9   PROGLUTYRLYSGLYGLUTRPLYSPROARG
10   GLNILEASPASNPROASPTYRLYSGLYTHR

Entity 2, ERp57 505 residues - Formula weight is not available

1   METARGLEUARGARGLEUALALEUPHEPRO
2   GLYVALALALEULEULEUALAALAALAARG
3   LEUALAALAALASERASPVALLEUGLULEU
4   THRASPASPASNPHEGLUSERARGILESER
5   ASPTHRGLYSERALAGLYLEUMETLEUVAL
6   GLUPHEPHEALAPROTRPCYSGLYHISCYS
7   LYSARGLEUALAPROGLUTYRGLUALAALA
8   ALATHRARGLEULYSGLYILEVALPROLEU
9   ALALYSVALASPCYSTHRALAASNTHRASN
10   THRCYSASNLYSTYRGLYVALSERGLYTYR
11   PROTHRLEULYSILEPHEARGASPGLYGLU
12   GLUALAGLYALATYRASPGLYPROARGTHR
13   ALAASPGLYILEVALSERHISLEULYSLYS
14   GLNALAGLYPROALASERVALPROLEUARG
15   THRGLUGLUGLUPHELYSLYSPHEILESER
16   ASPLYSASPALASERILEVALGLYPHEPHE
17   ASPASPSERPHESERGLUALAHISSERGLU
18   PHELEULYSALAALASERASNLEUARGASP
19   ASNTYRARGPHEALAHISTHRASNVALGLU
20   SERLEUVALASNGLUTYRASPASPASNGLY
21   GLUGLYILEILELEUPHEARGPROSERHIS
22   LEUTHRASNLYSPHEGLUASPLYSTHRVAL
23   ALATYRTHRGLUGLNLYSMETTHRSERGLY
24   LYSILELYSLYSPHEILEGLNGLUASNILE
25   PHEGLYILECYSPROHISMETTHRGLUASP
26   ASNLYSASPLEUILEGLNGLYLYSASPLEU
27   LEUILEALATYRTYRASPVALASPTYRGLU
28   LYSASNALALYSGLYSERASNTYRTRPARG
29   ASNARGVALMETMETVALALALYSLYSPHE
30   LEUASPALAGLYHISLYSLEUASNPHEALA
31   VALALASERARGLYSTHRPHESERHISGLU
32   LEUSERASPPHEGLYLEUGLUSERTHRALA
33   GLYGLUILEPROVALVALALAILEARGTHR
34   ALALYSGLYGLULYSPHEVALMETGLNGLU
35   GLUPHESERARGASPGLYLYSALALEUGLU
36   ARGPHELEUGLNASPTYRPHEASPGLYASN
37   LEULYSARGTYRLEULYSSERGLUPROILE
38   PROGLUSERASNASPGLYPROVALLYSVAL
39   VALVALALAGLUASNPHEASPGLUILEVAL
40   ASNASNGLUASNLYSASPVALLEUILEGLU
41   PHETYRALAPROTRPCYSGLYHISCYSLYS
42   ASNLEUGLUPROLYSTYRLYSGLULEUGLY
43   GLULYSLEUSERLYSASPPROASNILEVAL
44   ILEALALYSMETASPALATHRALAASNASP
45   VALPROSERPROTYRGLUVALARGGLYPHE
46   PROTHRILETYRPHESERPROALAASNLYS
47   LYSLEUASNPROLYSLYSTYRGLUGLYGLY
48   ARGGLULEUSERASPPHEILESERTYRLEU
49   GLNARGGLUALATHRASNPROPROVALILE
50   GLNGLUGLULYSPROLYSLYSLYSLYSLYS
51   ALAGLNGLUASPLEU

Samples:

sample_1: potassium dihydrogen phosphate 100 mM; sodium chloride 25 mM; beta-mercaptoethanol 10 mM; CRT, [U-15N, U2H], 0.14 mM; ERp57 0.38 mM

sample_2: potassium dihydrogen phosphate 100 mM; sodium chloride 25 mM; beta-mercaptoethanol 10 mM; CRT, [U-15N, U2H], 0.12 mM; ERp57 0.33 mM

sample_3: potassium dihydrogen phosphate 100 mM; sodium chloride 25 mM; beta-mercaptoethanol 10 mM; CRT, [U-15N, U2H], 0.11 mM; ERp57 0.29 mM

sample_4: potassium dihydrogen phosphate 100 mM; sodium chloride 25 mM; beta-mercaptoethanol 10 mM; CRT, [U-15N, U2H], 0.1 mM; ERp57 0.26 mM

sample_5: potassium dihydrogen phosphate 100 mM; sodium chloride 25 mM; beta-mercaptoethanol 10 mM; CRT, [U-15N, U2H], 0.09 mM; ERp57 0.24 mM

sample_6: potassium dihydrogen phosphate 100 mM; sodium chloride 25 mM; beta-mercaptoethanol 10 mM; CRT, [U-15N, U2H], 0.28 mM

sample_7: potassium dihydrogen phosphate 100 mM; sodium chloride 25 mM; beta-mercaptoethanol 10 mM; CRT, [U-15N, U2H], 0.14 mM; ERp57 0.07 mM

sample_conditions_1: pH: 7.0; pressure: ambient atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N TROSYsample_1isotropicsample_conditions_1
1H-15N TROSYsample_2isotropicsample_conditions_1
1H-15N TROSYsample_3isotropicsample_conditions_1
1H-15N TROSYsample_4isotropicsample_conditions_1
1H-15N TROSYsample_5isotropicsample_conditions_1
1H-15N TROSYsample_6isotropicsample_conditions_1
1H-15N TROSYsample_7isotropicsample_conditions_1

Software:

PROSA, Gintert, P., Dotsch, V., Wider,G . & WiithrichK, . - processing

XEASY, Bartels et al. - data analysis

NMR spectrometers:

  • Bruker DRX 750 MHz

Related Database Links:

PDB
DBJ BAA11345 BAC35852 BAD96780 BAE01267 BAE22866 BAA03759 BAA11928 BAI45467 BAK62544
EMBL CAA33053 CAA37446 CAA55890 CAG33351 CAA89996 CAH90193
GB AAA31188 AAA36582 AAA37569 AAA51916 AAB20096 AAB37397 AAC50331 AAC51518 AAH14433 AAH36000
REF NP_001075704 NP_001167604 NP_001231051 NP_001248060 NP_001274539 NP_001127250 NP_001162437 NP_001182645 NP_001233381 NP_005304
SP P14211 P15253 P18418 P27797 P28491 P30101 Q4VIT4 Q5RDG4
PRF 2201310A 2209333A
AlphaFold P14211 P15253 P18418 P27797 P28491 P30101 Q5RDG4 Q4VIT4