BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17240

Title: INFLUENZA HEMEGGLUTININ FUSION PEPTIDE MUTANT G13A   PubMed: 20826788

Deposition date: 2010-10-05 Original release date: 2010-10-19

Authors: Lai, Alex; Tamm, Lukas

Citation: Lai, Alex; Tamm, Lukas. "Shallow Boomerang-shaped Influenza Hemagglutinin G13A Mutant Structure Promotes Leaky Membrane Fusion."  J. Biol. Chem. 285, 37467-37475 (2010).

Assembly members:
Influenza_HA_fusion_peptide_G13A, polymer, 27 residues, Formula weight is not available

Natural source:   Common Name: Influenza A virus   Taxonomy ID: 11320   Superkingdom: virus   Kingdom: not available   Genus/species: Influenzavirus A not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET31b+

Entity Sequences (FASTA):
Influenza_HA_fusion_peptide_G13A: GLFGAIAGFIENAWEGMIDG GCGKKKK

Data sets:
Data typeCount
1H chemical shifts117

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Influenza HA fusion peptide(G13A)1

Entities:

Entity 1, Influenza HA fusion peptide(G13A) 27 residues - Formula weight is not available

1   GLYLEUPHEGLYALAILEALAGLYPHEILE
2   GLUASNALATRPGLUGLYMETILEASPGLY
3   GLYCYSGLYLYSLYSLYSLYS

Samples:

FP_N15: Influenza HA fusion peptide G13A, [U-15N], 1 mM; DPC, [U-2H], 200 mM; acetic acid, [U-2H], 20 mM; DTT 5 mM; H2O 95%; D2O 5%

FP: Influenza HA fusion peptide G13A, [U-15N], 2 mM; DPC, [U-2H], 200 mM; acetic acid, [U-2H], 20 mM; DTT 5 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYFPisotropicsample_conditions_1
2D 1H-1H NOESYFPisotropicsample_conditions_1
3D HNHAFP_N15isotropicsample_conditions_1
3D 1H-15N NOESYFP_N15isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

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