BMRB Entry 17263

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PDB ID: 2l52
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17263
MolProbity Validation Chart

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Title:
Solution structure of the small archaeal modifier protein 1 (SAMP1) from Methanosarcina acetivorans.
Deposition date:
2010-10-24
Original release date:
2011-01-18
Authors:
Damberger, Fred; Ranjan, Namit; Sutter, Markus; Allain, Frederic; Weber-Ban, Eilika
Citation:

Citation: Ranjan, Namit; Damberger, Fred; Sutter, Markus; Allain, Frederic H-T; Weber-Ban, Eilika. "Solution structure and activation mechanism of ubiquitin-like small archaeal modifier proteins."  J. Mol. Biol. 405, 1040-1055 (2011).
PubMed: 21112336

Assembly members:

Assembly members:
Small archaeal modifier protein 1 from Methanosarcina acetivorans, polymer, 99 residues, 10708.505 Da.

Natural source:

Natural source:   Common Name: Methanosarcina acetivorans   Taxonomy ID: 2214   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanosarcina acetivorans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pROEX (Invitrogen)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Small archaeal modifier protein 1 from Methanosarcina acetivorans: GHMAEVKVKLFANLREAAGT PELPLSGEKVIDVLLSLTDK YPALKYVIFEKGDEKSEILI LCGSINILINGNNIRHLEGL ETLLKDSDEIGILPPVSGG

Data sets:
Data typeCount
13C chemical shifts443
15N chemical shifts101
1H chemical shifts734
H exchange protection factors41
H exchange rates41
heteronuclear NOE values76

Time Domain Data

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Small archaeal modifier protein 1 from Methanosarcina acetivorans1

Entities:

Entity 1, Small archaeal modifier protein 1 from Methanosarcina acetivorans 99 residues - 10708.505 Da.

The first two residues at the N-terminus represent the remainder of a His-Tag cleaved by a protease and are not part of the native sequence.

1   GLYHISMETALAGLUVALLYSVALLYSLEU
2   PHEALAASNLEUARGGLUALAALAGLYTHR
3   PROGLULEUPROLEUSERGLYGLULYSVAL
4   ILEASPVALLEULEUSERLEUTHRASPLYS
5   TYRPROALALEULYSTYRVALILEPHEGLU
6   LYSGLYASPGLULYSSERGLUILELEUILE
7   LEUCYSGLYSERILEASNILELEUILEASN
8   GLYASNASNILEARGHISLEUGLUGLYLEU
9   GLUTHRLEULEULYSASPSERASPGLUILE
10   GLYILELEUPROPROVALSERGLYGLY

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