BMRB Entry 17271

Name: Co-ordinates and 1H, 13C and 15N chemical shift assignments for the complex of GPS2 53-90 and SMRT 167-207
Published: 2011-01-18

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PDB ID: 2l5g
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17271
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Co-ordinates and 1H, 13C and 15N chemical shift assignments for the complex of GPS2 53-90 and SMRT 167-207

Deposition date:

2010-11-01

Original release date:

2011-01-18

Authors:

Oberoi, Jasmeen; Yang, Ji-Chun; Neuhaus, David; Schwabe, John

Citation:

Citation: Oberoi, Jasmeen; Fairall, Louise; Watson, Peter; Yang, Ji-Chun; Czimmerer, Zsolt; Kampmann, Thorsten; Goult, Benjamin; Greenwood, Jacquie; Gooch, John; Kallenberger, Bettina; Nagy, Laszlo; Neuhaus, David; Schwabe, John. "Structural basis for the assembly of the SMRT/NCoR core transcriptional repression machinery." Nat. Struct. Mol. Biol. 18, 177-184 (2011).
PubMed: 21240272

Assembly members:

Assembly members:
GPS2_53-90, polymer, 38 residues, 4692.534 Da.
SMRT_167-207, polymer, 42 residues, 4948.712 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET13a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GPS2_53-90: KKEMEERMSLEETKEQILKL EEKLLALQEEKHQLFLQL
SMRT_167-207: GLSKEELIQNMDRVDREITM VEQQISKLKKKQQQLEEEAA KP

Data sets:

assigned_chemical_shifts
- GPS2_assignments

Data type	Count
13C chemical shifts	232
15N chemical shifts	90
1H chemical shifts	578

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GPS2_53-90	1
2	SMRT_167-207	2

Entities:

Entity 1, GPS2_53-90 38 residues - 4692.534 Da.

1

LYS

GLU

MET

GLU

ARG

MET

SER

LEU

2

GLU

THR

LYS

GLU

GLN

ILE

LEU

LYS

LEU

3

GLU

LYS

LEU

ALA

LEU

GLN

GLU

4

LYS

HIS

GLN

LEU

PHE

LEU

GLN

LEU

Entity 2, SMRT_167-207 42 residues - 4948.712 Da.

1

GLY

LEU

SER

LYS

GLU

LEU

ILE

GLN

ASN

2

MET

ASP

ARG

VAL

ASP

ARG

GLU

ILE

THR

MET

3

VAL

GLU

GLN

ILE

SER

LYS

LEU

LYS

4

LYS

GLN

LEU

GLU

ALA

5

LYS

PRO

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC CT aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC CT aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY (filtered to remove 15N-coupled signals in F1 and F2)	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY filtered to retain only interchain cross-peaks	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_3	isotropic	sample_conditions_1
2D 1H-1H NOESY filtered to retain only interchain cross-peaks	sample_3	isotropic	sample_conditions_1
3D HNHAHB	sample_1	isotropic	sample_conditions_1

PDB	2L5G 2L5G
DBJ	BAG63313 BAD92326 BAG72661
EMBL	CAB59255 CAG46524 CAG46550 CAJ82276
GB	AAB60432 AAH13652 AAI03902 AAI03903 AAI03904 AAD20944 AAD20946 AAD22973 AAI29557 AAI56550
REF	NP_001069242 NP_004480 XP_001365129 XP_002718857 XP_002748019 NP_001007033 NP_001038041 NP_001070729 NP_001084492 NP_001101804
SP	Q13227 Q9WU42 Q9Y618
TPG	DAA18863 DAA20733
AlphaFold	Q13227 Q9WU42 Q9Y618

BMRB Entry 17271

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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