BMRB Entry 17293
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17293
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Title: Solution structure of human J-protein co-chaperone Dph4 PubMed: 22367199
Deposition date: 2010-11-11 Original release date: 2012-01-09
Authors: Thakur, Anushikha; Chitoor, Balasubramanyam; Atreya, Hanudatta; Silva, Patrick
Citation: Thakur, Anushikha; Chitoor, Balasubramanyam; Goswami, Arvind; Pareek, Gautam; Atreya, Hanudatta. "Structure and mechanistic insights into novel iron-mediated moonlighting functions of human J-protein cochaperone, Dph4." J. Biol. Chem. 287, 13194-13205 (2012).
Assembly members:
Dph4, polymer, 155 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3A
Entity Sequences (FASTA):
Dph4: MMAVEQMPKKDWYSILGADP
SANISDLKQKYQKLILMYHP
DKQSTDVPAGTVEECVQKFI
EIDQAWKILGNEETKREYDL
QRCEDDLRNVGPVDAQVYLE
EMSWNEGDHSFYLSCRCGGK
YSVSKDEAEEVSLISCDTCS
LIIELLHYNHHHHHH
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 524 |
15N chemical shifts | 135 |
1H chemical shifts | 932 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Dph4 | 1 |
2 | Zn | 2 |
Entities:
Entity 1, Dph4 155 residues - Formula weight is not available
1 | MET | MET | ALA | VAL | GLU | GLN | MET | PRO | LYS | LYS | ||||
2 | ASP | TRP | TYR | SER | ILE | LEU | GLY | ALA | ASP | PRO | ||||
3 | SER | ALA | ASN | ILE | SER | ASP | LEU | LYS | GLN | LYS | ||||
4 | TYR | GLN | LYS | LEU | ILE | LEU | MET | TYR | HIS | PRO | ||||
5 | ASP | LYS | GLN | SER | THR | ASP | VAL | PRO | ALA | GLY | ||||
6 | THR | VAL | GLU | GLU | CYS | VAL | GLN | LYS | PHE | ILE | ||||
7 | GLU | ILE | ASP | GLN | ALA | TRP | LYS | ILE | LEU | GLY | ||||
8 | ASN | GLU | GLU | THR | LYS | ARG | GLU | TYR | ASP | LEU | ||||
9 | GLN | ARG | CYS | GLU | ASP | ASP | LEU | ARG | ASN | VAL | ||||
10 | GLY | PRO | VAL | ASP | ALA | GLN | VAL | TYR | LEU | GLU | ||||
11 | GLU | MET | SER | TRP | ASN | GLU | GLY | ASP | HIS | SER | ||||
12 | PHE | TYR | LEU | SER | CYS | ARG | CYS | GLY | GLY | LYS | ||||
13 | TYR | SER | VAL | SER | LYS | ASP | GLU | ALA | GLU | GLU | ||||
14 | VAL | SER | LEU | ILE | SER | CYS | ASP | THR | CYS | SER | ||||
15 | LEU | ILE | ILE | GLU | LEU | LEU | HIS | TYR | ASN | HIS | ||||
16 | HIS | HIS | HIS | HIS | HIS |
Entity 2, Zn - Zn - 65.409 Da.
1 | ZN |
Samples:
sample_1: Dph4 polypeptide, [U-13C; U-15N], 1 mM; Zn 1 mM; sodium chloride 80 mM; TRIS 20 mM; H2O 90%; D2O 10%
sample_2: Dph4 polypeptide 1 mM; Zn 1 mM; sodium chloride 80 mM; TRIS 20 mM; D2O 100%
sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D H(CCO)NH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D (H)C(CO)NH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CBCACO)NH | sample_1 | isotropic | sample_conditions_1 |
GFT (3,2)D HA(CA)CO(N)H | sample_1 | isotropic | sample_conditions_1 |
GFT (4,3)D NOESY-HCCH | sample_2 | isotropic | sample_conditions_1 |
Software:
XEASY, Bartels et al. - chemical shift assignment, peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing
xwinnmr, Bruker Biospin - collection
CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure solution
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 500 MHz
Related Database Links:
PDB | |
DBJ | BAF82354 |
GB | AAH36571 AAH63804 AIC62319 EAW68242 EAW68243 |
REF | NP_859057 XP_001141859 XP_003254415 XP_003830465 XP_009244778 |
SP | Q6P3W2 |
AlphaFold | Q6P3W2 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts