BMRB Entry 17350

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17350

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Title:
SOLUTION STRUCTURE OF THE R2 DOMAIN OF TALIN
Deposition date:
2010-12-07
Original release date:
2013-02-04
Authors:
Goult, Benjamin; Gingras, Alexandre; Bate, Neil; Roberts, Gordon; Barsukov, Igor; Critchley, David
Citation:

Citation: Goult, Benjamin; Zacharchenko, Tom; Bate, Neil; Tsang, Ricky; Hey, Fiona; Gingras, Alexandre; Elliott, Paul; Roberts, Gordon; Ballestrem, Christoph; Critchley, David; Barsukov, Igor. "RIAM and vinculin binding to talin are mutually exclusive and regulate adhesion assembly and turnover"  J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
vbs2a, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet151TOPO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
vbs2a: GIDPFTIGESDTDPHFQDVL MQLANAVASAAAALVLKAKS VAQRTEDSGLQTQVIAAATQ CALSTSQLVACTKVVAPTIS SPVCQEQLVEAGRLVAKAVE GCVSASQAATEDGQLLRGVG AAATAVTQALNELLQHVK

Data sets:
Data typeCount
13C chemical shifts396
15N chemical shifts131
1H chemical shifts131

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1vbs2a1

Entities:

Entity 1, vbs2a 138 residues - Formula weight is not available

Residues 1-6 (residues 649-654) represent a non-native affinity tag

1   GLYILEASPPROPHETHRILEGLYGLUSER
2   ASPTHRASPPROHISPHEGLNASPVALLEU
3   METGLNLEUALAASNALAVALALASERALA
4   ALAALAALALEUVALLEULYSALALYSSER
5   VALALAGLNARGTHRGLUASPSERGLYLEU
6   GLNTHRGLNVALILEALAALAALATHRGLN
7   CYSALALEUSERTHRSERGLNLEUVALALA
8   CYSTHRLYSVALVALALAPROTHRILESER
9   SERPROVALCYSGLNGLUGLNLEUVALGLU
10   ALAGLYARGLEUVALALALYSALAVALGLU
11   GLYCYSVALSERALASERGLNALAALATHR
12   GLUASPGLYGLNLEULEUARGGLYVALGLY
13   ALAALAALATHRALAVALTHRGLNALALEU
14   ASNGLULEULEUGLNHISVALLYS

Related Database Links:

PDB 1SJ8
DBJ BAA82979 BAC65702 BAE27781 BAG09941
EMBL CAA39588
GB AAD13152 AAF23322 AAF27330 AAH42923 AAI50811
PRF 1617167A
REF NP_001034114 NP_001192357 NP_006280 NP_035732 XP_001084941
SP P26039 Q9Y490
TPG DAA26829
AlphaFold P26039 Q9Y490

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