BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17356

Title: Biophysical studies of lipid interacting regions of DGD2 in Arabidopsis thaliana   PubMed: 21506606

Deposition date: 2010-12-10 Original release date: 2011-05-05

Authors: Szpryngiel, Scarlett; Ge, Changrong; Iakovleva, Irina; Lind, Jesper; Wieslander, Ake; Maler, Lena

Citation: Szpryngiel, Scarlett; Ge, Changrong; Iakovleva, Irina; Georgiev, Alexander; Lind, Jesper; Wieslander, Ake; Maler, Lena. "Lipid Interacting Regions in Phosphate Stress Glycosyltransferase atDGD2 from Arabidopsis thaliana."  Biochemistry 50, 4451-4466 (2011).

Assembly members:
atDGD2_S227_245, polymer, 19 residues, 2227.622 Da.

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
atDGD2_S227_245: QPFTKGAYYIGKMVWSKGY

Data sets:
Data typeCount
1H chemical shifts112

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DGD21

Entities:

Entity 1, DGD2 19 residues - 2227.622 Da.

1   GLNPROPHETHRLYSGLYALATYRTYRILE
2   GLYLYSMETVALTRPSERLYSGLYTYR

Samples:

sample_1: atDGD2_S227_245 0.5 mM; DPC, [U-2H], 50 mM; D2O, [U-2H], 10%; sodium phosphate 50 mM; H2O 90%

sample_conditions_1: ionic strength: 50 mM; pH: 5.7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance 500 MHz