BMRB Entry 17375
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17375
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Title: Assignment of Human Proliferating Cell Nuclear Antigen Complexed with a 20 Amino Acids Peptide from the C-terminal Region of p21 (WAF1/CIP1) PubMed: 23139781
Deposition date: 2010-12-22 Original release date: 2012-11-26
Authors: Lopez-Alonso, Jorge; Sanchez, Ricardo; Blanco, Francisco; Campos-Olivas, Ramon
Citation: De Biasio, Alfredo; Campos-Olivas, Ramon; Sanchez, Ricardo; Lopez-Alonso, Jorge; Pantoja-Uceda, David; Merino, Nekane; Villate, Maider; Martin-Garcia, Jose; Castillo, Francisco; Luque, Irene; Blanco, Francisco. "Proliferating Cell Nuclear Antigen (PCNA) Interactions in Solution Studied by NMR" PLoS One 7, e48390-e48390 (2012).
Assembly members:
hPCNA, polymer, 264 residues, Formula weight is not available
p2120, polymer, 20 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Rosetta BL21 (DE3)
Entity Sequences (FASTA):
hPCNA: GPHMFEARLVQGSILKKVLE
ALKDLINEACWDISSSGVNL
QSMDSSHVSLVQLTLRSEGF
DTYRCDRNLAMGVNLTSMSK
ILKCAGNEDIITLRAEDNAD
TLALVFEAPNQEKVSDYEMK
LMDLDVEQLGIPEQEYSCVV
KMPSGEFARICRDLSHIGDA
VVISCAKDGVKFSASGELGN
GNIKLSQTSNVDKEEEAVTI
EMNEPVQLTFALRYLNFFTK
ATPLSSTVTLSMSADVPLVV
EYKIADMGHLKYYLAPKIED
EEGS
p2120: KRRQTSMTDFYHSKRRLIFS
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 261 |
| 1H chemical shifts | 277 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hPCNA monomer 1 | 1 |
| 2 | hPCNA monomer 2 | 1 |
| 3 | hPCNA monomer 3 | 1 |
| 4 | P2120 molecule 1 | 2 |
| 5 | P2120 molecule 2 | 2 |
| 6 | P2120 molecule 3 | 2 |
Entities:
Entity 1, hPCNA monomer 1 264 residues - Formula weight is not available
Residues -3 to -1 are a non-native tag. The native sequence starts at residue number 4: MF...
| 1 | GLY | PRO | HIS | MET | PHE | GLU | ALA | ARG | LEU | VAL | ||||
| 2 | GLN | GLY | SER | ILE | LEU | LYS | LYS | VAL | LEU | GLU | ||||
| 3 | ALA | LEU | LYS | ASP | LEU | ILE | ASN | GLU | ALA | CYS | ||||
| 4 | TRP | ASP | ILE | SER | SER | SER | GLY | VAL | ASN | LEU | ||||
| 5 | GLN | SER | MET | ASP | SER | SER | HIS | VAL | SER | LEU | ||||
| 6 | VAL | GLN | LEU | THR | LEU | ARG | SER | GLU | GLY | PHE | ||||
| 7 | ASP | THR | TYR | ARG | CYS | ASP | ARG | ASN | LEU | ALA | ||||
| 8 | MET | GLY | VAL | ASN | LEU | THR | SER | MET | SER | LYS | ||||
| 9 | ILE | LEU | LYS | CYS | ALA | GLY | ASN | GLU | ASP | ILE | ||||
| 10 | ILE | THR | LEU | ARG | ALA | GLU | ASP | ASN | ALA | ASP | ||||
| 11 | THR | LEU | ALA | LEU | VAL | PHE | GLU | ALA | PRO | ASN | ||||
| 12 | GLN | GLU | LYS | VAL | SER | ASP | TYR | GLU | MET | LYS | ||||
| 13 | LEU | MET | ASP | LEU | ASP | VAL | GLU | GLN | LEU | GLY | ||||
| 14 | ILE | PRO | GLU | GLN | GLU | TYR | SER | CYS | VAL | VAL | ||||
| 15 | LYS | MET | PRO | SER | GLY | GLU | PHE | ALA | ARG | ILE | ||||
| 16 | CYS | ARG | ASP | LEU | SER | HIS | ILE | GLY | ASP | ALA | ||||
| 17 | VAL | VAL | ILE | SER | CYS | ALA | LYS | ASP | GLY | VAL | ||||
| 18 | LYS | PHE | SER | ALA | SER | GLY | GLU | LEU | GLY | ASN | ||||
| 19 | GLY | ASN | ILE | LYS | LEU | SER | GLN | THR | SER | ASN | ||||
| 20 | VAL | ASP | LYS | GLU | GLU | GLU | ALA | VAL | THR | ILE | ||||
| 21 | GLU | MET | ASN | GLU | PRO | VAL | GLN | LEU | THR | PHE | ||||
| 22 | ALA | LEU | ARG | TYR | LEU | ASN | PHE | PHE | THR | LYS | ||||
| 23 | ALA | THR | PRO | LEU | SER | SER | THR | VAL | THR | LEU | ||||
| 24 | SER | MET | SER | ALA | ASP | VAL | PRO | LEU | VAL | VAL | ||||
| 25 | GLU | TYR | LYS | ILE | ALA | ASP | MET | GLY | HIS | LEU | ||||
| 26 | LYS | TYR | TYR | LEU | ALA | PRO | LYS | ILE | GLU | ASP | ||||
| 27 | GLU | GLU | GLY | SER |
Entity 2, P2120 molecule 1 20 residues - Formula weight is not available
| 1 | LYS | ARG | ARG | GLN | THR | SER | MET | THR | ASP | PHE | |
| 2 | TYR | HIS | SER | LYS | ARG | ARG | LEU | ILE | PHE | SER |
Samples:
sample_1: hPCNA, [U-13C; U-15N], 0.5 mM; p2120 0.6 mM; sodium chloride 137 mM; potassium chloride 2.7 mM; sodium phosphate 10 mM; potassium phosphate 2 mM; H2O 93%; D2O 7%
sample_conditions_1: ionic strength: 0.327 M; pH: 7.0; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView v5.0.20, Johnson, One Moon Scientific - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
TOPSPIN, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
| BMRB | 15501 17376 |
| PDB | |
| DBJ | BAB28355 BAB28557 BAC40240 BAE30230 BAE30376 BAA23168 BAD93118 BAE88691 BAG10980 BAG61011 |
| EMBL | CAA40938 CAA68261 CAG38740 CAG46598 CAG38770 CAH25729 CAL37589 CAL37798 |
| GB | AAA35736 AAA60040 AAG10077 AAG37435 AAH00491 AAA16109 AAA19811 AAA85641 AAB29246 AAB59559 |
| REF | NP_001029666 NP_001233697 NP_001253019 NP_001274649 NP_001278854 NP_000380 NP_001009865 NP_001181651 NP_001207706 NP_001207707 |
| SP | P04961 P12004 P17918 P57761 P61258 O19002 P38936 |
| TPG | DAA23435 |
| PIR | S39358 |
| PRF | 2002363A |
| AlphaFold | P12004 P57761 P61258 P17918 P04961 P38936 O19002 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts