BMRB Entry 17386

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17386

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Title:
RBM10 RanBP2 zinc finger backbone chemical shifts
Deposition date:
2010-12-23
Original release date:
2011-01-28
Authors:
Mansfield, Robyn; Mackay, Joel; Nguyen, Cuong
Citation:

Citation: Nguyen, Cuong; Mansfield, Robyn; Leung, Wilfred; Vaz, Paula; Loughlin, Fionna; Grant, Richard; Mackay, Joel. "Characterization of a Family of RanBP2-Type Zinc Fingers that Can Recognize Single-Stranded RNA."  J. Mol. Biol. 407, 273-283 (2011).
PubMed: 21256132

Assembly members:

Assembly members:
RBM10_ZnF, polymer, 41 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RBM10_ZnF: GSSDPKPKINEDWLCNKCGV QNFKRREKCFKCGVPKSEAE Q

Data sets:
Data typeCount
13C chemical shifts76
15N chemical shifts33
1H chemical shifts33

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1protein component1
2zinc ion2

Entities:

Entity 1, protein component 41 residues - Formula weight is not available

N-terminal GS is non-native, resulting from cleavage of a GST tag.

1   GLYSERSERASPPROLYSPROLYSILEASN
2   GLUASPTRPLEUCYSASNLYSCYSGLYVAL
3   GLNASNPHELYSARGARGGLULYSCYSPHE
4   LYSCYSGLYVALPROLYSSERGLUALAGLU
5   GLN

Entity 2, zinc ion - Zn - 65.409 Da.

1   ZN

Related Database Links:

BMRB 25433
PDB 2MXV
DBJ BAA09471 BAA12144 BAC31087 BAC40753 BAC65490
EMBL CAD97933 CAL37951
GB AAH00681 AAH03089 AAH04181 AAH04674 AAH08733
REF NP_001161247 NP_001161248 NP_001191395 NP_001191396 NP_001191397
SP P70501 P98175 Q99KG3
TPG DAA12902 DAA12903
AlphaFold P70501 P98175 Q99KG3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks