BMRB Entry 17387

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17387

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Title:
RBM5 RanBP2 zinc finger backbone chemical shifts
Deposition date:
2010-12-23
Original release date:
2011-01-28
Authors:
Mansfield, Robyn; Nguyen, Cuong; Mackay, Joel
Citation:

Citation: Nguyen, Cuong; Mansfield, Robyn; Leung, Wilfred; Vaz, Paula; Loughlin, Fionna; Grant, Richard; Mackay, Joel. "Characterization of a Family of RanBP2-Type Zinc Fingers that Can Recognize Single-Stranded RNA."  J. Mol. Biol. 407, 273-283 (2011).
PubMed: 21256132

Assembly members:

Assembly members:
RBM5_ZnF, polymer, 40 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RBM5_ZnF: GSSNPRPKFEDWLCNKCCLN NFRKRLKCFRCGADKFDSEQ

Data sets:
Data typeCount
13C chemical shifts69
15N chemical shifts33
1H chemical shifts33

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1protein component1
2zinc ion2

Entities:

Entity 1, protein component 40 residues - Formula weight is not available

N-terminal GS is non-native, resulting from cleavage of a GST tag.

1   GLYSERSERASNPROARGPROLYSPHEGLU
2   ASPTRPLEUCYSASNLYSCYSCYSLEUASN
3   ASNPHEARGLYSARGLEULYSCYSPHEARG
4   CYSGLYALAASPLYSPHEASPSERGLUGLN

Entity 2, zinc ion - Zn - 65.409 Da.

1   ZN

Related Database Links:

BMRB 17969 17970
PDB 2LK0 2LK1
DBJ BAE22042 BAE27501 BAE87995 BAG36742 BAG59728
EMBL CAC69136
GB AAA99715 AAB42216 AAD04159 AAF02422 AAH19766
REF NP_001039839 NP_001094018 NP_001248103 NP_005769 NP_683732
SP B2GV05 P52756 Q1RMU5 Q91YE7
TPG DAA16943
AlphaFold B2GV05 P52756 Q1RMU5 Q91YE7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks