BMRB Entry 17417

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17417

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Title:
mutant C335A, K309C of the Nek2 kinase leucine zipper
Deposition date:
2011-01-20
Original release date:
2011-04-08
Authors:
Pfuhl, Mark
Citation:

Citation: Croasdale, Rebecca; Ivins, Frank; Muskett, Fred; Daviter, Tina; Scott, David; Hardy, Tara; Smerdon, Steven; Fry, Andrew; Pfuhl, Mark. "An undecided coiled coil: the leucine zipper of Nek2 kinase exhibits atypical conformational exchange dynamics."  J. Biol. Chem. 286, 27537-27547 (2011).
PubMed: 21669869

Assembly members:

Assembly members:
LZ5C335AK309C, polymer, 46 residues, 5438.1991 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LZ5C335AK309C: GAMAVLSELKLCEIQLQERE RALKAREERLEQKEQELAVR ERLAED

Data sets:
Data typeCount
13C chemical shifts88
15N chemical shifts46
1H chemical shifts186

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1LZ5, chain 11
2LZ5, chain 21

Entities:

Entity 1, LZ5, chain 1 46 residues - 5438.1991 Da.

residues 1-4 are a cloning artifact

1   GLYALAMETALAVALLEUSERGLULEULYS
2   LEUCYSGLUILEGLNLEUGLNGLUARGGLU
3   ARGALALEULYSALAARGGLUGLUARGLEU
4   GLUGLNLYSGLUGLNGLULEUALAVALARG
5   GLUARGLEUALAGLUASP

Related Database Links:

UNP NEK2_HUMAN

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