BMRB Entry 17434
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17434
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Title: solution structure of the C-terminal domain of H-NS like protein Bv3F PubMed: 21673140
Deposition date: 2011-01-29 Original release date: 2015-06-23
Authors: Li, Yifei; Xia, Bin
Citation: Gordon, Blair; Li, Yifei; Cote, Atina; Weirauch, Matthew; Ding, Pengfei; Hughes, Timothy; Navarre, William; Xia, Bin; Liu, Jun. "Structural basis for recognition of AT-rich DNA by unrelated xenogeneic silencing proteins" Proc. Natl. Acad. Sci. U. S. A. 108, 10690-10695 (2011).
Assembly members:
Bv3F, polymer, 44 residues, 4828.533 Da.
Natural source: Common Name: Burkholderia vietnamiensis Taxonomy ID: 60552 Superkingdom: Bacteria Kingdom: not available Genus/species: Burkholderia vietnamiensis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a
Entity Sequences (FASTA):
Bv3F: MSTVPKYRDPATGKTWSGRG
RQPAWLGNDPAAFLIQPDLP
AILE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 188 |
| 15N chemical shifts | 40 |
| 1H chemical shifts | 287 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Bv3F | 1 |
Entities:
Entity 1, Bv3F 44 residues - 4828.533 Da.
| 1 | MET | SER | THR | VAL | PRO | LYS | TYR | ARG | ASP | PRO | ||||
| 2 | ALA | THR | GLY | LYS | THR | TRP | SER | GLY | ARG | GLY | ||||
| 3 | ARG | GLN | PRO | ALA | TRP | LEU | GLY | ASN | ASP | PRO | ||||
| 4 | ALA | ALA | PHE | LEU | ILE | GLN | PRO | ASP | LEU | PRO | ||||
| 5 | ALA | ILE | LEU | GLU |
Samples:
sample_1: C-terminal domain of H-NS like protein Bv3F, [U-13C, >90%; U-15 N, >90%], 0.7 mM; sodium phosphate, [U-100% 13C; U-100% 15N], 50 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - geometry optimization
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts