BMRB Entry 17461

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17461

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Title:
Multiple Cycles of Global Unfolding of GroEL-bound Cyclophilin A Evidenced by NMR
Deposition date:
2011-02-11
Original release date:
2012-06-06
Authors:
Nieba-Axmann, Sabine; Ottiger, Marcel; Wuthrich, Kurt; Pluckthun, Andreas
Citation:

Citation: Nieba-Axmann, Sabine; Ottiger, Marcel; Wuthrich, Kurt; Pluckthun, Andreas. "Multiple Cycles of Global Unfolding of GroEL-bound Cyclophilin A Evidenced by NMR"  J. Mol. Biol. 271, 803-818 (1997).

Assembly members:

Assembly members:
GroEL, polymer, 548 residues, Formula weight is not available
CypA, polymer, 165 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pOF39

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GroEL: MAAKDVKFGNDARVKMLRGV NVLADAVKVTLGPKGRNVVL DKSFGAPTITKDGVSVAREI ELEDKFENMGAQMVKEVASK ANDAAGDGTTTATVLAQAII TEGLKAVAAGMNPMDLKRGI DKAVTVAVEELKALSVPCSD SKAIAQVGTISANSDETVGK LIAEAMDKVGKEGVITVEDG TGLQDELDVVEGMQFDRGYL SPYFINKPETGAVELESPFI LLADKKISNIREMLPVLEAV AKAGKPLLIIAEDVEGEALA TLVVNTMRGIVKVAAVKAPG FGDRRKAMLQDIATLTGGTV ISEEIGMELEKATLEDLGQA KRVVINKDTTTIIDGVGEEA AIQGRVAQIRQQIEEATSDY DREKLQERVAKLAGGVAVIK VGAATEVEMKEKKARVEDAL HATRAAVEEGVVAGGGVALI RVASKLADLRGQNEDQNVGI KVALRAMEAPLRQIVLNCGE EPSVVANTVKGGDGNYGYNA ATEEYGNMIDMGILDPTKVT RSALQYAASVAGLMITTECM VTDLPKNDAADLGAAGGMGG MGGMGGMM
CypA: MVNPTVFFDIAVDGEPLGRV SFELFADKVPKTAENFRALS TGEKGFGYKGSCFHRIIPGF MCQGGDFTRHNGTGGKSIYG EKFEDENFILKHTGPGILSM ANAGPNTNGSQFFICTAKTE WLDGKHVVFGKVKEGMNIVE AMERFGSRNGKTSKKITIAD CGQLE

Data typeCount
kinetic rates11

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1GroEL1
2CypA2

Entities:

Entity 1, GroEL 548 residues - Formula weight is not available

1   METALAALALYSASPVALLYSPHEGLYASN
2   ASPALAARGVALLYSMETLEUARGGLYVAL
3   ASNVALLEUALAASPALAVALLYSVALTHR
4   LEUGLYPROLYSGLYARGASNVALVALLEU
5   ASPLYSSERPHEGLYALAPROTHRILETHR
6   LYSASPGLYVALSERVALALAARGGLUILE
7   GLULEUGLUASPLYSPHEGLUASNMETGLY
8   ALAGLNMETVALLYSGLUVALALASERLYS
9   ALAASNASPALAALAGLYASPGLYTHRTHR
10   THRALATHRVALLEUALAGLNALAILEILE
11   THRGLUGLYLEULYSALAVALALAALAGLY
12   METASNPROMETASPLEULYSARGGLYILE
13   ASPLYSALAVALTHRVALALAVALGLUGLU
14   LEULYSALALEUSERVALPROCYSSERASP
15   SERLYSALAILEALAGLNVALGLYTHRILE
16   SERALAASNSERASPGLUTHRVALGLYLYS
17   LEUILEALAGLUALAMETASPLYSVALGLY
18   LYSGLUGLYVALILETHRVALGLUASPGLY
19   THRGLYLEUGLNASPGLULEUASPVALVAL
20   GLUGLYMETGLNPHEASPARGGLYTYRLEU
21   SERPROTYRPHEILEASNLYSPROGLUTHR
22   GLYALAVALGLULEUGLUSERPROPHEILE
23   LEULEUALAASPLYSLYSILESERASNILE
24   ARGGLUMETLEUPROVALLEUGLUALAVAL
25   ALALYSALAGLYLYSPROLEULEUILEILE
26   ALAGLUASPVALGLUGLYGLUALALEUALA
27   THRLEUVALVALASNTHRMETARGGLYILE
28   VALLYSVALALAALAVALLYSALAPROGLY
29   PHEGLYASPARGARGLYSALAMETLEUGLN
30   ASPILEALATHRLEUTHRGLYGLYTHRVAL
31   ILESERGLUGLUILEGLYMETGLULEUGLU
32   LYSALATHRLEUGLUASPLEUGLYGLNALA
33   LYSARGVALVALILEASNLYSASPTHRTHR
34   THRILEILEASPGLYVALGLYGLUGLUALA
35   ALAILEGLNGLYARGVALALAGLNILEARG
36   GLNGLNILEGLUGLUALATHRSERASPTYR
37   ASPARGGLULYSLEUGLNGLUARGVALALA
38   LYSLEUALAGLYGLYVALALAVALILELYS
39   VALGLYALAALATHRGLUVALGLUMETLYS
40   GLULYSLYSALAARGVALGLUASPALALEU
41   HISALATHRARGALAALAVALGLUGLUGLY
42   VALVALALAGLYGLYGLYVALALALEUILE
43   ARGVALALASERLYSLEUALAASPLEUARG
44   GLYGLNASNGLUASPGLNASNVALGLYILE
45   LYSVALALALEUARGALAMETGLUALAPRO
46   LEUARGGLNILEVALLEUASNCYSGLYGLU
47   GLUPROSERVALVALALAASNTHRVALLYS
48   GLYGLYASPGLYASNTYRGLYTYRASNALA
49   ALATHRGLUGLUTYRGLYASNMETILEASP
50   METGLYILELEUASPPROTHRLYSVALTHR
51   ARGSERALALEUGLNTYRALAALASERVAL
52   ALAGLYLEUMETILETHRTHRGLUCYSMET
53   VALTHRASPLEUPROLYSASNASPALAALA
54   ASPLEUGLYALAALAGLYGLYMETGLYGLY
55   METGLYGLYMETGLYGLYMETMET

Entity 2, CypA 165 residues - Formula weight is not available

1   METVALASNPROTHRVALPHEPHEASPILE
2   ALAVALASPGLYGLUPROLEUGLYARGVAL
3   SERPHEGLULEUPHEALAASPLYSVALPRO
4   LYSTHRALAGLUASNPHEARGALALEUSER
5   THRGLYGLULYSGLYPHEGLYTYRLYSGLY
6   SERCYSPHEHISARGILEILEPROGLYPHE
7   METCYSGLNGLYGLYASPPHETHRARGHIS
8   ASNGLYTHRGLYGLYLYSSERILETYRGLY
9   GLULYSPHEGLUASPGLUASNPHEILELEU
10   LYSHISTHRGLYPROGLYILELEUSERMET
11   ALAASNALAGLYPROASNTHRASNGLYSER
12   GLNPHEPHEILECYSTHRALALYSTHRGLU
13   TRPLEUASPGLYLYSHISVALVALPHEGLY
14   LYSVALLYSGLUGLYMETASNILEVALGLU
15   ALAMETGLUARGPHEGLYSERARGASNGLY
16   LYSTHRSERLYSLYSILETHRILEALAASP
17   CYSGLYGLNLEUGLU

Related Database Links:

PDB 1AON 1GR5 1GRL 1GRU 1J4Z 1KP8 1KPO 1MNF 1OEL 1PCQ 1PF9 1SS8 1SVT 1SX3 1SX4 1XCK 2C7C 2C7D 2C7E 2CGT 2EU1 2NWC 2YEY 2YNJ 3C9V 3CAU 3E76 3WVL 3ZPZ 3ZQ0 3ZQ1 4AAQ 4AAR 4AAS 4AAU 4AB2 4AB3 4HEL 4KI8 4PKN 4PKO 4WGL 4WSC 1AK4 1AWQ 1AWR 1AWS 1AWT 1AWU 1AWV 1BCK 1CWA 1CWB 1CWC 1CWF 1CWH 1CWI 1CWJ 1CWK 1CWL 1CWM 1CWO 1FGL 1M63 1M9C 1M9D 1M9E 1M9F 1M9X 1M9Y 1MF8 1MIK 1NMK 1OCA 1RMH 1VBS 1VBT 1W8L 1W8M 1W8V 1YND 1ZKF 2ALF 2CPL 2CYH 2RMA 2RMB 2WLW 2X25 2X2A 2X2C 2X2D 2X83 2XGY 3CYH 3CYS 3K0M 3K0N 3K0O 3K0P 3K0Q 3K0R 3ODI 3ODL 3RDD 4CYH 4DGA 4DGB 4DGC 4DGD 4DGE 4IPZ 5CYH
DBJ BAA25209 BAA25211 BAA25229 BAA94286 BAB38547 BAE01146 BAE30323 BAE87660 BAF82774 BAF83540
EMBL CAA30698 CAC16675 CAC18572 CAC18574 CAC18576 CAA34961 CAA37039 CAA68264 CAG32988 CAH91833
GB AAA85277 AAA97042 AAC77103 AAG59342 AAL09389 AAB81959 AAB81960 AAB81961 AAF69142 AAF78600
PIR AE1045 B86110 CSHYAC
PRF 1407243B 1503232A
REF NP_313151 NP_418567 NP_458769 NP_463194 NP_710008 NP_001009370 NP_001027981 NP_001126060 NP_001270275 NP_001271703
SP A1AJ51 A7ZV12 A8A7N9 A8AMQ6 A9MFR9 P14851 P62935 P62936 P62937 P62938
BMRB 17218 2208 25337 531 532
TPG DAA25853 DAA25854
AlphaFold A1AJ51 A7ZV12 A8A7N9 A8AMQ6 A9MFR9 P14851 P62935 P62936 P62937 P62938