BMRB Entry 17506
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17506
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Citation: Meng, Wenli; Raleigh, Daniel. "Analysis of electrostatic interactions in the denatured state ensemble of the N-terminal domain of L9 under native conditions." Proteins 79, 3500-3510 (2011).
PubMed: 21915914
Assembly members:
NTL9, polymer, 56 residues, Formula weight is not available
Natural source: Common Name: G. Stearothermophilus Taxonomy ID: 1422 Superkingdom: Bacteria Kingdom: not available Genus/species: Geobacillus Stearothermophilus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3a
Entity Sequences (FASTA):
NTL9: MKVIFLKDVKGKGKKGEIKN
VADGYANNFLFKQGLAIEAT
PANLKALEAQKQKEQR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 160 |
| 15N chemical shifts | 54 |
| 1H chemical shifts | 107 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | N-TERMINAL DOMAIN | 1 |
Entities:
Entity 1, N-TERMINAL DOMAIN 56 residues - Formula weight is not available
| 1 | MET | LYS | VAL | ILE | PHE | LEU | LYS | ASP | VAL | LYS | ||||
| 2 | GLY | LYS | GLY | LYS | LYS | GLY | GLU | ILE | LYS | ASN | ||||
| 3 | VAL | ALA | ASP | GLY | TYR | ALA | ASN | ASN | PHE | LEU | ||||
| 4 | PHE | LYS | GLN | GLY | LEU | ALA | ILE | GLU | ALA | THR | ||||
| 5 | PRO | ALA | ASN | LEU | LYS | ALA | LEU | GLU | ALA | GLN | ||||
| 6 | LYS | GLN | LYS | GLU | GLN | ARG |
Samples:
13C15Nlabel: NTL9, [U-100% 13C; U-100% 15N], 1 mM; sodium chloride 100 mM; sodium acetate 20 mM; H2O 90%; D2O 10%
15Nlabel: NTL9, [U-100% 15N], 1 mM; sodium chloride 100 mM; sodium acetate 20 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.12 M; pH: 5.5; pressure: 1 atm; temperature: 285 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | 13C15Nlabel | isotropic | sample_conditions_1 |
| 3D HNCACB | 13C15Nlabel | isotropic | sample_conditions_1 |
| 3D HNCO | 13C15Nlabel | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | 13C15Nlabel | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | 15Nlabel | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Bruker Avance 500 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks