BMRB Entry 17555

Name: Chemical shift assignments of the VBS1 domain of talin
Published: 2012-05-10

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17555

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Chemical shift assignments of the VBS1 domain of talin PubMed: 22351767

Deposition date: 2011-03-30 Original release date: 2012-05-10

Authors: Goult, Ben; Gingras, Alex; Bate, Neil; Banno, Asoka; Ginsberg, Mark; Roberts, Gordon; Barsukov, Igor; Critchley, David

Citation: Banno, Asoka; Goult, Benjamin; Lee, Hosup; Bate, Neil; Critchley, David; Ginsberg, Mark. "Subcellular Localization of Talin Is Regulated by Inter-domain Interactions." J. Biol. Chem. 287, 13799-13812 (2012).

Assembly members:
vbs1, polymer, 180 residues, Formula weight is not available

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet151

Entity Sequences (FASTA):
vbs1: GIDPFTRGHMPPLTSAQQAL TGTINSSMQAVQAAQATLDD FETLPPLGQDAASKAWRKNK MDESKHEIHSQVDAITAGTA SVVNLTAGDPAETDYTAVGC AVTTISSNLTEMSRGVKLLA ALLEDEGGNGRPLLQAAKGL AGAVSELLRSAQPASAEPRQ NLLQAAGNVGQASGELLQQI

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	324
15N chemical shifts	163
1H chemical shifts	162

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	vbs1	1

Entities:

Entity 1, vbs1 180 residues - Formula weight is not available

Residues 1-6 represent a non-native affinity tag

1	GLY	ILE	ASP	PRO	PHE	THR	ARG	GLY	HIS	MET
2	PRO	PRO	LEU	THR	SER	ALA	GLN	GLN	ALA	LEU
3	THR	GLY	THR	ILE	ASN	SER	SER	MET	GLN	ALA
4	VAL	GLN	ALA	ALA	GLN	ALA	THR	LEU	ASP	ASP
5	PHE	GLU	THR	LEU	PRO	PRO	LEU	GLY	GLN	ASP
6	ALA	ALA	SER	LYS	ALA	TRP	ARG	LYS	ASN	LYS
7	MET	ASP	GLU	SER	LYS	HIS	GLU	ILE	HIS	SER
8	GLN	VAL	ASP	ALA	ILE	THR	ALA	GLY	THR	ALA
9	SER	VAL	VAL	ASN	LEU	THR	ALA	GLY	ASP	PRO
10	ALA	GLU	THR	ASP	TYR	THR	ALA	VAL	GLY	CYS
11	ALA	VAL	THR	THR	ILE	SER	SER	ASN	LEU	THR
12	GLU	MET	SER	ARG	GLY	VAL	LYS	LEU	LEU	ALA
13	ALA	LEU	LEU	GLU	ASP	GLU	GLY	GLY	ASN	GLY
14	ARG	PRO	LEU	LEU	GLN	ALA	ALA	LYS	GLY	LEU
15	ALA	GLY	ALA	VAL	SER	GLU	LEU	LEU	ARG	SER
16	ALA	GLN	PRO	ALA	SER	ALA	GLU	PRO	ARG	GLN
17	ASN	LEU	LEU	GLN	ALA	ALA	GLY	ASN	VAL	GLY
18	GLN	ALA	SER	GLY	GLU	LEU	LEU	GLN	GLN	ILE

Samples:

sample_1: vbs1, [U-100% 13C; U-100% 15N], 0.8 ± 0.05 mM; D2O, [U-100% 2H], 10 ± 0.1 %; DTT 2 ± 0.01 mM; sodium phosphate 20 ± 0.1 mM; sodium chloride 50 ± 0.1 mM; H2O 90 ± 0.1 %

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

CCPNAnalysis v1.5, CCPN - chemical shift assignment, peak picking, processing

NMR spectrometers:

Bruker DRX 600 MHz

PDB	1SJ7 1SJ8
DBJ	BAA82979 BAC65702 BAE27781 BAG09941
EMBL	CAA39588
GB	AAD13152 AAF23322 AAF27330 AAH42923 AAI50811
PRF	1617167A
REF	NP_001034114 NP_001192357 NP_006280 NP_035732 XP_001084941
SP	P26039 Q9Y490
TPG	DAA26829
AlphaFold	P26039 Q9Y490

Biological Magnetic Resonance Data Bank