BMRB Entry 17563
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17563
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Title: Glycyl-tRNA(GCC) anticodon stem-loop from Bacillus subtilis
Deposition date: 2011-03-31 Original release date: 2012-04-04
Authors: Chang, Andrew; Nikonowicz, Edward
Citation: Chang, Andrew; Nikonowicz, Edward. "Structure solution of three glycyl-tRNA anticodon stem-loops" .
Assembly members:
glycyl-tRNA, polymer, 17 residues, 5434.313 Da.
Natural source: Common Name: B. subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: enzymatic semisynthesis Host organism: Bacillus subtilis
Entity Sequences (FASTA):
glycyl-tRNA: GGGCCUUGCCAAGGUCC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 90 |
| 1H chemical shifts | 108 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RNA (5'-R(*GP*GP*GP*CP*CP*UP*UP*GP*CP*CP*AP*AP*GP*GP*UP*CP*C)-3') | 1 |
Entities:
Entity 1, RNA (5'-R(*GP*GP*GP*CP*CP*UP*UP*GP*CP*CP*AP*AP*GP*GP*UP*CP*C)-3') 17 residues - 5434.313 Da.
| 1 | G | G | G | C | C | U | U | G | C | C | ||||
| 2 | A | A | G | G | U | C | C |
Samples:
sample_1: RNA, [U-98% 13C; U-98% 15N], 0.02 – 0.04 mM; D2O 100%; KH2PO4 10 mM; K2HPO4 10 mM; KCl 10 mM; EDTA 0.2 mM
sample_2: RNA0.02 – 0.04 mM; D2O 100%; KH2PO4 10 mM; K2HPO4 10 mM; KCl 10 mM; EDTA 0.2 mM
sample_conditions_1: ionic strength: 10 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D HCN | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
FELIX, Accelrys Software Inc. - chemical shift assignment, processing
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 600 MHz