BMRB Entry 17581
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17581
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Title: Solution structure of the skeletal muscle and neuronal voltage gated sodium channel antagonist mu-conotoxin CnIIIC PubMed: 22229737
Deposition date: 2011-04-08 Original release date: 2012-05-09
Authors: Favreau, P.; Benoit, E.; Hocking, Henry; Carlier, L.; D'hoedt, D.; Leipold, E.; Markgraf, R.; Schlumberger, S.; Cordova, M.; Gaertner, H.; Paolini-bertrand, M.; Hartley, O.; Tytgat, J.; Heinemann, S.; Bertrand, D.; Boelens, R.; Stocklin, R.; Molgo, J.
Citation: Favreau, Philippe; Benoit, Evelyne; Hocking, Henry; Carlier, Ludovic; Leipold, Dieter; Markgraf, Enrico; Schlumberger, Rene; Cordova, Sebastien; Gaertner, Marco; Paolini-Bertrand, Hubert; Hartley, Marianne; Tytgat, Oliver; Heinemann, Jan; Bertrand, Stefan; Boelens, Daniel; Stocklin, Rolf; Molgo, Reto. "A novel -conopeptide, CnIIIC, exerts potent and preferential inhibition of NaV1.2/1.4 channels and blocks neuronal nicotinic acetylcholine receptors." Br. J. Pharmacol. 166, 1654-1668 (2012).
Assembly members:
MU-CONOTOXIN_CNIIIC, polymer, 23 residues, 2378.7115 Da.
Natural source: Common Name: gastropods Taxonomy ID: 101297 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Conus consors
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
MU-CONOTOXIN_CNIIIC: EGCCNGPKGCSSKWCRDHAR
CCX
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 11 |
1H chemical shifts | 116 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | MU-CONOTOXIN CNIIIC | 1 |
Entities:
Entity 1, MU-CONOTOXIN CNIIIC 23 residues - 2378.7115 Da.
1 | GLU | GLY | CYS | CYS | ASN | GLY | PRO | LYS | GLY | CYS | ||||
2 | SER | SER | LYS | TRP | CYS | ARG | ASP | HIS | ALA | ARG | ||||
3 | CYS | CYS | PCA |
Samples:
sample_1: MU-CONOTOXIN_CNIIIC, [U-13C; U-15N], 1.3 mM
sample_conditions_1: ionic strength: 0.0 mM; pH: 4.000; pressure: 1 atm; temperature: 278.000 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
NOESY | sample_1 | solution | sample_conditions_1 |
DQF-COSY | sample_1 | solution | sample_conditions_1 |
e-COSY | sample_1 | solution | sample_conditions_1 |
TOCSY | sample_1 | solution | sample_conditions_1 |
Software:
AutoDep v4.3, PDBe - collection
CNS vany, BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- - refinement
CYANA vany, Guntert, Mumenthaler and Wuthrich - data analysis
SPARKY vany, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 750 MHz
Related Database Links:
PDB |