BMRB Entry 17593

Name: NMR analysis of TM1_TM2 in TFE:water(0.1%TFA) (1:1)
Published: 2012-08-07

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17593

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR analysis of TM1_TM2 in TFE:water(0.1%TFA) (1:1)

Deposition date:

2011-04-14

Original release date:

2012-08-07

Authors:

Cohen, Leah; Zerbe, Oliver; Naider, Fred; Arshava, Boris

Citation:

Citation: Cohen, Leah; Arshava, Boris; Neumoin, Alexey; Becker, Jeffrey; Geuntert, Peter; Zerbe, Oliver; Naider, Fred. "Comparative NMR analysis of an 80-residue G protein-coupled receptor fragment in two membrane mimetic environments" Biochim. Biophys. Acta 1808, 2674-2684 (2011).
PubMed: 21791199

Assembly members:

Assembly members:
TM1_TM2, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pLC01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TM1_TM2: GNGSTITFDELQGLVNSTVT QAILFGVRSGAAALTLIVVW ITSRSRKTPIFIINQVSLFL IILHSALYFKYLLSNYSSVT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	313
15N chemical shifts	89
1H chemical shifts	575

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TM1_TM2	1

Entities:

Entity 1, TM1_TM2 80 residues - Formula weight is not available

1	GLY	ASN	GLY	SER	THR	ILE	THR	PHE	ASP	GLU
2	LEU	GLN	GLY	LEU	VAL	ASN	SER	THR	VAL	THR
3	GLN	ALA	ILE	LEU	PHE	GLY	VAL	ARG	SER	GLY
4	ALA	ALA	ALA	LEU	THR	LEU	ILE	VAL	VAL	TRP
5	ILE	THR	SER	ARG	SER	ARG	LYS	THR	PRO	ILE
6	PHE	ILE	ILE	ASN	GLN	VAL	SER	LEU	PHE	LEU
7	ILE	ILE	LEU	HIS	SER	ALA	LEU	TYR	PHE	LYS
8	TYR	LEU	LEU	SER	ASN	TYR	SER	SER	VAL	THR

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_3	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_3	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D H(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_4	isotropic	sample_conditions_1
3D 1H-13C TOCSY	sample_4	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_6	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_6	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_4	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_7	isotropic	sample_conditions_1
3D 1H-13C HSQC-NOESY-HSQC	sample_5	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_5	isotropic	sample_conditions_1
HMCM(CBCA)NH_leu/ile	sample_5	isotropic	sample_conditions_1
HMCM(CA)NH_val	sample_5	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_5	isotropic	sample_conditions_1

BMRB	15995 17211
PDB	2K9P

BMRB Entry 17593

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	ASN	GLY	SER	THR	ILE	THR	PHE	ASP	GLU
2	LEU	GLN	GLY	LEU	VAL	ASN	SER	THR	VAL	THR
3	GLN	ALA	ILE	LEU	PHE	GLY	VAL	ARG	SER	GLY
4	ALA	ALA	ALA	LEU	THR	LEU	ILE	VAL	VAL	TRP
5	ILE	THR	SER	ARG	SER	ARG	LYS	THR	PRO	ILE
6	PHE	ILE	ILE	ASN	GLN	VAL	SER	LEU	PHE	LEU
7	ILE	ILE	LEU	HIS	SER	ALA	LEU	TYR	PHE	LYS
8	TYR	LEU	LEU	SER	ASN	TYR	SER	SER	VAL	THR

1	GLY	ASN	GLY	SER	THR	ILE	THR	PHE	ASP	GLU
2	LEU	GLN	GLY	LEU	VAL	ASN	SER	THR	VAL	THR
3	GLN	ALA	ILE	LEU	PHE	GLY	VAL	ARG	SER	GLY
4	ALA	ALA	ALA	LEU	THR	LEU	ILE	VAL	VAL	TRP
5	ILE	THR	SER	ARG	SER	ARG	LYS	THR	PRO	ILE
6	PHE	ILE	ILE	ASN	GLN	VAL	SER	LEU	PHE	LEU
7	ILE	ILE	LEU	HIS	SER	ALA	LEU	TYR	PHE	LYS
8	TYR	LEU	LEU	SER	ASN	TYR	SER	SER	VAL	THR

1	GLY	ASN	GLY	SER	THR	ILE	THR	PHE	ASP	GLU
2	LEU	GLN	GLY	LEU	VAL	ASN	SER	THR	VAL	THR
3	GLN	ALA	ILE	LEU	PHE	GLY	VAL	ARG	SER	GLY
4	ALA	ALA	ALA	LEU	THR	LEU	ILE	VAL	VAL	TRP
5	ILE	THR	SER	ARG	SER	ARG	LYS	THR	PRO	ILE
6	PHE	ILE	ILE	ASN	GLN	VAL	SER	LEU	PHE	LEU
7	ILE	ILE	LEU	HIS	SER	ALA	LEU	TYR	PHE	LYS
8	TYR	LEU	LEU	SER	ASN	TYR	SER	SER	VAL	THR