BMRB Entry 17665

Name: human alpha synuclein construct
Published: 2011-10-19

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17665

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

human alpha synuclein construct

Deposition date:

2011-05-25

Original release date:

2011-10-19

Authors:

Pochapsky, Thomas; Perovic, Iva

Citation:

Citation: Wang, Wei; Perovic, Iva; Chittuluru, Johnathan; Kaganovich, Alice; Nguyen, Linh; Liao, Jingling; Auclair, Jared; Johnson, Derrick; Landeru, Anuradha; Simorellis, Alana; Ju, Shulin; Cookson, Mark; Asturias, Francisco; Agar, Jeffrey; Webb, Brian; Kang, Chulhee; Ringe, Dagmar; Petsko, Gregory; Pochapsky, Thomas; Hoang, Quyen. "A soluble -synuclein construct forms a dynamic tetramer." Proc. Natl. Acad. Sci. U.S.A. 108, 17797-17802 (2011).
PubMed: 22006323

Assembly members:

Assembly members:
aSyn, polymer, 150 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: N/A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
aSyn: GPLGSPEFPGMDVFMKGLSK AKEGVVAAAEKTKQGVAEAA GKTKEGVLYVGSKTKEGVVH GVATVAEKTKEQVTNVGGAV VTGVTAVAQKTVEGAGSIAA ATGFVKKDQLGKNEEGAPQE GILEDMPVDPDNEAYEMPSE EGYQDYEPEA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	260
15N chemical shifts	134
1H chemical shifts	660

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	alpha synuclein, chain 1	1
2	alpha synuclein, chain 2	1
3	alpha synuclein, chain 3	1
4	alpha synuclein, chain 4	1

Entities:

Entity 1, alpha synuclein, chain 1 150 residues - Formula weight is not available

1	GLY	PRO	LEU	GLY	SER	PRO	GLU	PHE	PRO	GLY
2	MET	ASP	VAL	PHE	MET	LYS	GLY	LEU	SER	LYS
3	ALA	LYS	GLU	GLY	VAL	VAL	ALA	ALA	ALA	GLU
4	LYS	THR	LYS	GLN	GLY	VAL	ALA	GLU	ALA	ALA
5	GLY	LYS	THR	LYS	GLU	GLY	VAL	LEU	TYR	VAL
6	GLY	SER	LYS	THR	LYS	GLU	GLY	VAL	VAL	HIS
7	GLY	VAL	ALA	THR	VAL	ALA	GLU	LYS	THR	LYS
8	GLU	GLN	VAL	THR	ASN	VAL	GLY	GLY	ALA	VAL
9	VAL	THR	GLY	VAL	THR	ALA	VAL	ALA	GLN	LYS
10	THR	VAL	GLU	GLY	ALA	GLY	SER	ILE	ALA	ALA
11	ALA	THR	GLY	PHE	VAL	LYS	LYS	ASP	GLN	LEU
12	GLY	LYS	ASN	GLU	GLU	GLY	ALA	PRO	GLN	GLU
13	GLY	ILE	LEU	GLU	ASP	MET	PRO	VAL	ASP	PRO
14	ASP	ASN	GLU	ALA	TYR	GLU	MET	PRO	SER	GLU
15	GLU	GLY	TYR	GLN	ASP	TYR	GLU	PRO	GLU	ALA

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
15N-filtered 2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1

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CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 17665

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	GLY	PRO	LEU	GLY	SER	PRO	GLU	PHE	PRO	GLY
2	MET	ASP	VAL	PHE	MET	LYS	GLY	LEU	SER	LYS
3	ALA	LYS	GLU	GLY	VAL	VAL	ALA	ALA	ALA	GLU
4	LYS	THR	LYS	GLN	GLY	VAL	ALA	GLU	ALA	ALA
5	GLY	LYS	THR	LYS	GLU	GLY	VAL	LEU	TYR	VAL
6	GLY	SER	LYS	THR	LYS	GLU	GLY	VAL	VAL	HIS
7	GLY	VAL	ALA	THR	VAL	ALA	GLU	LYS	THR	LYS
8	GLU	GLN	VAL	THR	ASN	VAL	GLY	GLY	ALA	VAL
9	VAL	THR	GLY	VAL	THR	ALA	VAL	ALA	GLN	LYS
10	THR	VAL	GLU	GLY	ALA	GLY	SER	ILE	ALA	ALA
11	ALA	THR	GLY	PHE	VAL	LYS	LYS	ASP	GLN	LEU
12	GLY	LYS	ASN	GLU	GLU	GLY	ALA	PRO	GLN	GLU
13	GLY	ILE	LEU	GLU	ASP	MET	PRO	VAL	ASP	PRO
14	ASP	ASN	GLU	ALA	TYR	GLU	MET	PRO	SER	GLU
15	GLU	GLY	TYR	GLN	ASP	TYR	GLU	PRO	GLU	ALA

1	GLY	PRO	LEU	GLY	SER	PRO	GLU	PHE	PRO	GLY
2	MET	ASP	VAL	PHE	MET	LYS	GLY	LEU	SER	LYS
3	ALA	LYS	GLU	GLY	VAL	VAL	ALA	ALA	ALA	GLU
4	LYS	THR	LYS	GLN	GLY	VAL	ALA	GLU	ALA	ALA
5	GLY	LYS	THR	LYS	GLU	GLY	VAL	LEU	TYR	VAL
6	GLY	SER	LYS	THR	LYS	GLU	GLY	VAL	VAL	HIS
7	GLY	VAL	ALA	THR	VAL	ALA	GLU	LYS	THR	LYS
8	GLU	GLN	VAL	THR	ASN	VAL	GLY	GLY	ALA	VAL
9	VAL	THR	GLY	VAL	THR	ALA	VAL	ALA	GLN	LYS
10	THR	VAL	GLU	GLY	ALA	GLY	SER	ILE	ALA	ALA
11	ALA	THR	GLY	PHE	VAL	LYS	LYS	ASP	GLN	LEU
12	GLY	LYS	ASN	GLU	GLU	GLY	ALA	PRO	GLN	GLU
13	GLY	ILE	LEU	GLU	ASP	MET	PRO	VAL	ASP	PRO
14	ASP	ASN	GLU	ALA	TYR	GLU	MET	PRO	SER	GLU
15	GLU	GLY	TYR	GLN	ASP	TYR	GLU	PRO	GLU	ALA

1	GLY	PRO	LEU	GLY	SER	PRO	GLU	PHE	PRO	GLY
2	MET	ASP	VAL	PHE	MET	LYS	GLY	LEU	SER	LYS
3	ALA	LYS	GLU	GLY	VAL	VAL	ALA	ALA	ALA	GLU
4	LYS	THR	LYS	GLN	GLY	VAL	ALA	GLU	ALA	ALA
5	GLY	LYS	THR	LYS	GLU	GLY	VAL	LEU	TYR	VAL
6	GLY	SER	LYS	THR	LYS	GLU	GLY	VAL	VAL	HIS
7	GLY	VAL	ALA	THR	VAL	ALA	GLU	LYS	THR	LYS
8	GLU	GLN	VAL	THR	ASN	VAL	GLY	GLY	ALA	VAL
9	VAL	THR	GLY	VAL	THR	ALA	VAL	ALA	GLN	LYS
10	THR	VAL	GLU	GLY	ALA	GLY	SER	ILE	ALA	ALA
11	ALA	THR	GLY	PHE	VAL	LYS	LYS	ASP	GLN	LEU
12	GLY	LYS	ASN	GLU	GLU	GLY	ALA	PRO	GLN	GLU
13	GLY	ILE	LEU	GLU	ASP	MET	PRO	VAL	ASP	PRO
14	ASP	ASN	GLU	ALA	TYR	GLU	MET	PRO	SER	GLU
15	GLU	GLY	TYR	GLN	ASP	TYR	GLU	PRO	GLU	ALA