BMRB Entry 17707
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17707
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Daubner, Gerrit; Clery, Antoine; Jayne, Sandrine; Stevenin, James; Allain, Frederic H-T. "A syn-anti conformational difference allows SRSF2 to recognize guanines and cytosines equally well." EMBO J. 31, 162-174 (2012).
PubMed: 22002536
Assembly members:
SRSF2_RRM, polymer, 135 residues, 11705.210 Da.
RNA_(5'-R(*UP*GP*GP*AP*GP*U)-3')_, polymer, 6 residues, 1932.211 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28a
Entity Sequences (FASTA):
SRSF2_RRM: MGSSHHHHHHSSGLVPRGSH
MASMTGGQQMGRGSMSYGRP
PPDVEGMTSLKVDNLTYRTS
PDTLRRVFEKYGRVGDVYIP
RDRYTKESRGFAFVRFHDKR
DAEDAMDAMDGAVLDGRELR
VQMARYGRPPDSHHS
RNA_(5'-R(*UP*GP*GP*AP*GP*U)-3')_: UGGAGU
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 268 |
| 15N chemical shifts | 97 |
| 1H chemical shifts | 669 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SRSF2_RRM | 1 |
| 2 | RNA (5'-R(*UP*GP*GP*AP*GP*U)-3') | 2 |
Entities:
Entity 1, SRSF2_RRM 135 residues - 11705.210 Da.
Residues 1-34 present the HIS and T7 Tag; Residues 35-131 the RRM domain.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | ALA | SER | MET | THR | GLY | GLY | GLN | GLN | MET | ||||
| 4 | GLY | ARG | GLY | SER | MET | SER | TYR | GLY | ARG | PRO | ||||
| 5 | PRO | PRO | ASP | VAL | GLU | GLY | MET | THR | SER | LEU | ||||
| 6 | LYS | VAL | ASP | ASN | LEU | THR | TYR | ARG | THR | SER | ||||
| 7 | PRO | ASP | THR | LEU | ARG | ARG | VAL | PHE | GLU | LYS | ||||
| 8 | TYR | GLY | ARG | VAL | GLY | ASP | VAL | TYR | ILE | PRO | ||||
| 9 | ARG | ASP | ARG | TYR | THR | LYS | GLU | SER | ARG | GLY | ||||
| 10 | PHE | ALA | PHE | VAL | ARG | PHE | HIS | ASP | LYS | ARG | ||||
| 11 | ASP | ALA | GLU | ASP | ALA | MET | ASP | ALA | MET | ASP | ||||
| 12 | GLY | ALA | VAL | LEU | ASP | GLY | ARG | GLU | LEU | ARG | ||||
| 13 | VAL | GLN | MET | ALA | ARG | TYR | GLY | ARG | PRO | PRO | ||||
| 14 | ASP | SER | HIS | HIS | SER |
Entity 2, RNA (5'-R(*UP*GP*GP*AP*GP*U)-3') 6 residues - 1932.211 Da.
| 1 | U | G | G | A | G | U |
Samples:
SRSF2_RRM_+_UGGAGU_sample_1: SRSF2 RRM, [U-15N], 0.75 ± 0.1 mM; UGGAGU1, [U-15N], 0.75 ± 0.1 mM; SRSF2 RRM, [U-13C; U-15N], 0.75 ± 0.1 mM; UGGAGU2, [U-13C; U-15N], 0.75 ± 0.1 mM; L-Arg 50 mM; L-Glu 50 mM; NaH2PO4 20 mM; H2O 90%; D2O 10%
SRSF2_RRM_+_UGGAGU_D2O: SRSF2 RRM, [U-15N], 0.75 ± 0.1 mM; UGGAGU1, [U-15N], 0.75 ± 0.1 mM; L-Arg 50 mM; L-Glu 50 mM; NaH2PO4 20 mM; D2O 100%
SRSF2_RRM_+_UGGAGU_sample_conditions: ionic strength: 120 mM; pH: 5.5; pressure: 1 atm; temperature: 310.8 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | SRSF2_RRM_+_UGGAGU_sample_1 | isotropic | SRSF2_RRM_+_UGGAGU_sample_conditions |
| 2D 1H-13C HSQC | SRSF2_RRM_+_UGGAGU_sample_1 | isotropic | SRSF2_RRM_+_UGGAGU_sample_conditions |
| 3D HCACO | SRSF2_RRM_+_UGGAGU_sample_1 | isotropic | SRSF2_RRM_+_UGGAGU_sample_conditions |
| 3D HNCO | SRSF2_RRM_+_UGGAGU_sample_1 | isotropic | SRSF2_RRM_+_UGGAGU_sample_conditions |
| 3D HNCA | SRSF2_RRM_+_UGGAGU_sample_1 | isotropic | SRSF2_RRM_+_UGGAGU_sample_conditions |
| 3D HN(CO)CA | SRSF2_RRM_+_UGGAGU_sample_1 | isotropic | SRSF2_RRM_+_UGGAGU_sample_conditions |
| 3D CBCA(CO)NH | SRSF2_RRM_+_UGGAGU_sample_1 | isotropic | SRSF2_RRM_+_UGGAGU_sample_conditions |
| 3D HNCACB | SRSF2_RRM_+_UGGAGU_sample_1 | isotropic | SRSF2_RRM_+_UGGAGU_sample_conditions |
| 3D H(CCO)NH | SRSF2_RRM_+_UGGAGU_sample_1 | isotropic | SRSF2_RRM_+_UGGAGU_sample_conditions |
| 3D HCCH-TOCSY | SRSF2_RRM_+_UGGAGU_sample_1 | isotropic | SRSF2_RRM_+_UGGAGU_sample_conditions |
| 3D 1H-13C NOESY | SRSF2_RRM_+_UGGAGU_sample_1 | isotropic | SRSF2_RRM_+_UGGAGU_sample_conditions |
| 3D 1H-13C NOESY | SRSF2_RRM_+_UGGAGU_sample_1 | isotropic | SRSF2_RRM_+_UGGAGU_sample_conditions |
| 3D 1H-15N NOESY | SRSF2_RRM_+_UGGAGU_sample_1 | isotropic | SRSF2_RRM_+_UGGAGU_sample_conditions |
| 2D 1H-1H NOESY | SRSF2_RRM_+_UGGAGU_D2O | isotropic | SRSF2_RRM_+_UGGAGU_sample_conditions |
| 2D 1H-1H TOCSY | SRSF2_RRM_+_UGGAGU_D2O | isotropic | SRSF2_RRM_+_UGGAGU_sample_conditions |
Software:
CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution
AMBER v9, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 600 MHz
- Bruker Avance 700 MHz
- Bruker Avance 900 MHz
Related Database Links:
| BMRB | 16444 17705 17706 |
| PDB | |
| DBJ | BAC03903 BAC04206 BAC36346 BAC39610 BAC40111 |
| EMBL | CAA44306 CAA44307 CAA53383 CAA67134 CAJ82901 |
| GB | AAA60306 AAC71000 AAH00339 AAH01303 AAH05493 |
| PIR | A42701 |
| PRF | 1805195A 1805195B |
| REF | NP_001001305 NP_001009720 NP_001029290 NP_001029490 NP_001070697 |
| SP | P30352 Q01130 Q06A98 Q3MHR5 Q5R1W5 |
| TPG | DAA18198 |
| AlphaFold | P30352 Q01130 Q06A98 Q3MHR5 Q5R1W5 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks