BMRB Entry 17715
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17715
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Title: Itch WW domain 2
Deposition date: 2011-06-16 Original release date: 2019-08-29
Authors: Barbato, Gaetano; Bellomaria, Alessia; Melino, Sonia
Citation: Bellomaria, Alessia; Melino, Sonia; Barbato, Gaetano; Paci, Maurizio; Melino, Gerry. "Itch-E3 ligase second WW domain 1H-13C-15N assignments" Biomol. NMR Assignments ., .-..
Assembly members:
Itch_WW2, polymer, 49 residues, 5000 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: none
Entity Sequences (FASTA):
Itch_WW2: GPLGSDRPEPLPPGWERRVD
NMGRIYYVDHFTRTTTWQRP
TLESVRNYE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 187 |
| 15N chemical shifts | 44 |
| 1H chemical shifts | 267 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | WW2 | 1 |
Entities:
Entity 1, WW2 49 residues - 5000 Da.
| 1 | GLY | PRO | LEU | GLY | SER | ASP | ARG | PRO | GLU | PRO | ||||
| 2 | LEU | PRO | PRO | GLY | TRP | GLU | ARG | ARG | VAL | ASP | ||||
| 3 | ASN | MET | GLY | ARG | ILE | TYR | TYR | VAL | ASP | HIS | ||||
| 4 | PHE | THR | ARG | THR | THR | THR | TRP | GLN | ARG | PRO | ||||
| 5 | THR | LEU | GLU | SER | VAL | ARG | ASN | TYR | GLU |
Samples:
sample_1: Itch WW2, [U-100% 13C; U-100% 15N], 0.6 mM; potassium chloride 50 mM; NaPi 10 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView vNMRViewJ, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific - chemical shift assignment, processing
NMR spectrometers:
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts