BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17755

Title: 1H, 13C, and 15N resonance assignments of human regenerating family I-alpha protein (Reg I-alpha)   PubMed: 21918816

Deposition date: 2011-07-01 Original release date: 2011-10-12

Authors: Ho, Meng-Ru; Chen, Chinpan

Citation: Ho, Meng-Ru; Chen, Chinpan. "1H, 13C, and 15N resonance assignments for human regenerating family I protein."  Biomol. NMR Assignments 6, 135-137 (2012).

Assembly members:
Human_Reg_I-alpha, polymer, 132 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE1

Entity Sequences (FASTA):
Human_Reg_I-alpha: SCPEGTNAYRSYCYYFNEDR ETWVDADLYCQNMNSGNLVS VLTQAEGAFVASLIKESGTD DFNVWIGLHDPKKNRRWHWS SGSLVSYKSWGIGAPSSVNP GYCVSLTSSTGFQKWKDVPC EDKFSFVCKFKN

Data sets:
Data typeCount
13C chemical shifts552
15N chemical shifts144
1H chemical shifts837

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Reg I-alpha monomer1

Entities:

Entity 1, Reg I-alpha monomer 132 residues - Formula weight is not available

1   SERCYSPROGLUGLYTHRASNALATYRARG
2   SERTYRCYSTYRTYRPHEASNGLUASPARG
3   GLUTHRTRPVALASPALAASPLEUTYRCYS
4   GLNASNMETASNSERGLYASNLEUVALSER
5   VALLEUTHRGLNALAGLUGLYALAPHEVAL
6   ALASERLEUILELYSGLUSERGLYTHRASP
7   ASPPHEASNVALTRPILEGLYLEUHISASP
8   PROLYSLYSASNARGARGTRPHISTRPSER
9   SERGLYSERLEUVALSERTYRLYSSERTRP
10   GLYILEGLYALAPROSERSERVALASNPRO
11   GLYTYRCYSVALSERLEUTHRSERSERTHR
12   GLYPHEGLNLYSTRPLYSASPVALPROCYS
13   GLUASPLYSPHESERPHEVALCYSLYSPHE
14   LYSASN

Samples:

sample_1: Reg I-alpha, [U-100% 13C; U-100% 15N; U-80% 2H], 0.5 – 1.0 mM; NaH2PO4 20 mM; sodium chloride 50 mM; potassium chloride 50 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 50 mM; pH: 4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAF84670 BAI45866
GB AAA36558 AAA36559 AAA60546 AAD51330 AAH05350
PRF 1617122A
REF NP_002900 XP_001163554 XP_002811895 XP_003282186 XP_003816658
SP P05451
AlphaFold P05451

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts