BMRB Entry 17826

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17826

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Title:
Galectin-7
Deposition date:
2011-08-03
Original release date:
2011-09-09
Authors:
Nesmelova, Irina; Moreno, Manuel; Miller, Michelle; Andre, Sabine; Jimenez-Barbero, Jesus; Gabius, Hans-Joachim; Mayo, Kevin
Citation:

Citation: Nesmelova, Irina; Berbis, Manuel Alvaro; Miller, Michelle; Canada, F. Javier; Andre, Sabine; Jimenez-Barbero, Jesus; Gabius, Hans-Joachim; Mayo, Kevin. "1H, 13C, and 15N backbone and side-chain chemical shift assignments for the 31 kDa human galectin-7 (p53-induced gene 1) homodimer, a pro-apoptotic lectin."  Biomol. NMR Assignments 6, 127-129 (2012).
PubMed: 21898049

Assembly members:

Assembly members:
gal-7, polymer, 135 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE-60/hGal-7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
gal-7: SNVPHKSSLPEGIRPGTVLR IRGLVPPNASRFHVNLLCGE EQGSDAALHFNPRLDTSEVV FNSKEQGSWGREERGPGVPF QRGQPFEVLIIASDDGFKAV VGDAQYHHFRHRLPLARVRL VEVGGDVQLDSVRIF

Data sets:
Data typeCount
13C chemical shifts1045
15N chemical shifts264
1H chemical shifts1624

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1galectin-7, chain 11
2galectin-7, chain 21

Entities:

Entity 1, galectin-7, chain 1 135 residues - Formula weight is not available

1   SERASNVALPROHISLYSSERSERLEUPRO
2   GLUGLYILEARGPROGLYTHRVALLEUARG
3   ILEARGGLYLEUVALPROPROASNALASER
4   ARGPHEHISVALASNLEULEUCYSGLYGLU
5   GLUGLNGLYSERASPALAALALEUHISPHE
6   ASNPROARGLEUASPTHRSERGLUVALVAL
7   PHEASNSERLYSGLUGLNGLYSERTRPGLY
8   ARGGLUGLUARGGLYPROGLYVALPROPHE
9   GLNARGGLYGLNPROPHEGLUVALLEUILE
10   ILEALASERASPASPGLYPHELYSALAVAL
11   VALGLYASPALAGLNTYRHISHISPHEARG
12   HISARGLEUPROLEUALAARGVALARGLEU
13   VALGLUVALGLYGLYASPVALGLNLEUASP
14   SERVALARGILEPHE

Samples:

sample_1: gal-7, [U-100% 13C; U-100% 15N], 1.0 – 1.5 mM; H2O 90%; D2O 10%; potassium phosphate 20 mM

sample_2: gal-7, [U-100% 13C; U-100% 15N], 0.7 – 1.5 mM; D2O 100%; potassium phosphate 20 mM

sample_conditions_1: ionic strength: 20 mM; pH: 7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Varian INOVA 800 MHz
  • Bruker Avance 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAJ21133
EMBL CAG33198
GB AAA67899 AAA86820 AAH42911 AAH61588 AAH73743
REF NP_001035972 NP_002298 XP_001083444 XP_003282512 XP_003812310
SP P47929
AlphaFold P47929

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks