BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17916

Title: Backbone assignments for the C-terminal domain of yeast Tfg2   PubMed: 22095626

Deposition date: 2011-09-06 Original release date: 2011-12-14

Authors: Kilpatrick, Adina; Koharudin, Leonardus; Gronenborn, Angela

Citation: Kilpatrick, Adina; Koharudin, Leonardus; Calero, Guillermo; Gronenborn, Angela. "Structural and binding studies of the C-terminal domains of yeast TFIIF subunits Tfg1 and Tfg2."  Proteins ., .-. (2011).

Assembly members:
Tfg2, polymer, 93 residues, Formula weight is not available

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-18

Entity Sequences (FASTA):
Tfg2: MASKSNIKSIRMPKKEILDY LFKLFDEYDYWSLKGLKERT RQPEAHLKECLDKVATLVKK GPYAFKYTLRPEYKKLKEEE RKATLGELADEQT

Data sets:
Data typeCount
13C chemical shifts270
15N chemical shifts89
1H chemical shifts176

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tfg21

Entities:

Entity 1, Tfg2 93 residues - Formula weight is not available

Residues 1-3 represent a non-native affinity tag.

1   METALASERLYSSERASNILELYSSERILE
2   ARGMETPROLYSLYSGLUILELEUASPTYR
3   LEUPHELYSLEUPHEASPGLUTYRASPTYR
4   TRPSERLEULYSGLYLEULYSGLUARGTHR
5   ARGGLNPROGLUALAHISLEULYSGLUCYS
6   LEUASPLYSVALALATHRLEUVALLYSLYS
7   GLYPROTYRALAPHELYSTYRTHRLEUARG
8   PROGLUTYRLYSLYSLEULYSGLUGLUGLU
9   ARGLYSALATHRLEUGLYGLULEUALAASP
10   GLUGLNTHR

Samples:

sample_1: Tfg2, [U-98% 13C; U-98% 15N], 1 – 1.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ GAA23391
EMBL CAA96988 CAY79765
GB AAA61642 AAT92890 AHY79373 AJP38791 AJR76111
REF NP_011519
SP P41896
TPG DAA08103
AlphaFold P41896

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts