BMRB Entry 17934

Name: AIDA1 PTB domain
Published: 2012-09-17

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PDB ID: 2m38
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17934
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: AIDA1 PTB domain PubMed: 23799029

Deposition date: 2011-09-12 Original release date: 2012-09-17

Authors: Donaldson, Logan

Citation: Smirnova, Ekaterina; Shanbhag, Riya; Kurabi, Arwa; Mobli, Mehdi; Kwan, Jamie; Donaldson, Logan. "Solution structure and peptide binding of the PTB domain from the AIDA1 postsynaptic signaling scaffolding protein." PLoS ONE 8, e65605-e65605 (2013).

Assembly members:
AIDA1 PTB domain, polymer, 153 residues, 15011.303 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28

Entity Sequences (FASTA):
AIDA1 PTB domain: STPVQAWQHHPEKLIAQSCD YKAAYLGSMLIKELRGTEST QDACAKMRANCQKSTEQMKK VPTIILSVSAKGVKFIDATN KNIIAEHEIRNISCAAQDPE DLSTFAYITKDLKSNHHYCH VFTAFDVNLAAEIILTLGQA FEVAYQLALQARK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	561
15N chemical shifts	134
1H chemical shifts	858

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AIDA1 PTB domain	1

Entities:

Entity 1, AIDA1 PTB domain 153 residues - 15011.303 Da.

1

SER

THR

PRO

VAL

GLN

ALA

TRP

GLN

HIS

2

PRO

GLU

LYS

LEU

ILE

ALA

GLN

SER

CYS

ASP

3

TYR

LYS

ALA

TYR

LEU

GLY

SER

MET

LEU

4

ILE

LYS

GLU

LEU

ARG

GLY

THR

GLU

SER

THR

5

GLN

ASP

ALA

CYS

ALA

LYS

MET

ARG

ALA

ASN

6

CYS

GLN

LYS

SER

THR

GLU

GLN

MET

LYS

7

VAL

PRO

THR

ILE

LEU

SER

VAL

SER

ALA

8

LYS

GLY

VAL

LYS

PHE

ILE

ASP

ALA

THR

ASN

9

LYS

ASN

ILE

ALA

GLU

HIS

GLU

ILE

ARG

10

ASN

ILE

SER

CYS

ALA

GLN

ASP

PRO

GLU

11

ASP

LEU

SER

THR

PHE

ALA

TYR

ILE

THR

LYS

12

ASP

LEU

LYS

SER

ASN

HIS

TYR

CYS

HIS

13

VAL

PHE

THR

ALA

PHE

ASP

VAL

ASN

LEU

ALA

14

ALA

GLU

ILE

LEU

THR

LEU

GLY

GLN

ALA

15

PHE

GLU

VAL

ALA

TYR

GLN

LEU

ALA

LEU

GLN

16

ALA

ARG

LYS

Samples:

sample_1: AIDA1 PTB domain 0.8 mM; sodium azide 0.05%; sodium chloride 150 mM; sodium phosphate 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

CYANA v3, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

Varian Uniform NMR System 600 MHz
Bruker Avance 900 MHz

BMRB	17428
PDB	2M38
DBJ	BAE87950
GB	EDL21516 EDL21517 EDL21518 EDM16964 EDM16965
REF	XP_003126745 XP_005658157 XP_006077488 XP_013846696

Biological Magnetic Resonance Data Bank