BMRB Entry 18116
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18116
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Title: backbone and sidechain order parameters for ubiquitin at 1, 400, 800, 1200, 1600 and 2500 bar PubMed: 22452540
Deposition date: 2011-12-01 Original release date: 2019-08-29
Authors: Fu, Yinan; Kasinath, Vignesh; Moorman, Veronica; Nucci, Nathaniel; Hilser, Vincent; Wand, A
Citation: Fu, Yinan; Kasinath, Vignesh; Moorman, Veronica; Nucci, Nathaniel; Hilser, Vincent; Wand, A Joshua. "Coupled motion in proteins revealed by pressure perturbation" J. Am. Chem. Soc. 134, 8543-8550 (2012).
Assembly members:
ubiquitin, polymer, 76 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b
Entity Sequences (FASTA):
ubiquitin: MQIFVKTLTGKTITLEVEPS
DTIENVKAKIQDKEGIPPDQ
QRLIFAGKQLEDGRTLSDYN
IQKESTLHLVLRLRGG
Data type | Count |
order parameters | 546 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | ubiquitin | 1 |
Entities:
Entity 1, ubiquitin 76 residues - Formula weight is not available
1 | MET | GLN | ILE | PHE | VAL | LYS | THR | LEU | THR | GLY | ||||
2 | LYS | THR | ILE | THR | LEU | GLU | VAL | GLU | PRO | SER | ||||
3 | ASP | THR | ILE | GLU | ASN | VAL | LYS | ALA | LYS | ILE | ||||
4 | GLN | ASP | LYS | GLU | GLY | ILE | PRO | PRO | ASP | GLN | ||||
5 | GLN | ARG | LEU | ILE | PHE | ALA | GLY | LYS | GLN | LEU | ||||
6 | GLU | ASP | GLY | ARG | THR | LEU | SER | ASP | TYR | ASN | ||||
7 | ILE | GLN | LYS | GLU | SER | THR | LEU | HIS | LEU | VAL | ||||
8 | LEU | ARG | LEU | ARG | GLY | GLY |
Samples:
sample_1: ubiquitin, [U-100% 13C; U-55% 2H], 1.2 mM; ubiquitin, [U-100% 15N], 0.8 mM; sodium chloride 50 mM; sodium acetate 50 mM; sodium azide 3 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 100 mM; pH: 5.0; pressure: 1 bar; temperature: 273 K
sample_conditions_2: ionic strength: 100 mM; pH: 5.0; pressure: 400 bar; temperature: 273 K
sample_conditions_3: ionic strength: 100 mM; pH: 5.0; pressure: 800 bar; temperature: 273 K
sample_conditions_4: ionic strength: 100 mM; pH: 5.0; pressure: 1200 bar; temperature: 273 K
sample_conditions_5: ionic strength: 100 mM; pH: 5.0; pressure: 1600 bar; temperature: 273 K
sample_conditions_6: ionic strength: 100 mM; pH: 5.0; pressure: 2500 bar; temperature: 273 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
15N relaxation | sample_1 | isotropic | sample_conditions_1 |
2H relaxation | sample_1 | isotropic | sample_conditions_1 |
15N relaxation | sample_1 | isotropic | sample_conditions_2 |
2H relaxation | sample_1 | isotropic | sample_conditions_2 |
15N relaxation | sample_1 | isotropic | sample_conditions_3 |
2H relaxation | sample_1 | isotropic | sample_conditions_3 |
15N relaxation | sample_1 | isotropic | sample_conditions_4 |
2H relaxation | sample_1 | isotropic | sample_conditions_4 |
15N relaxation | sample_1 | isotropic | sample_conditions_5 |
2H relaxation | sample_1 | isotropic | sample_conditions_5 |
15N relaxation | sample_1 | isotropic | sample_conditions_6 |
2H relaxation | sample_1 | isotropic | sample_conditions_6 |
Software:
FELIX, Accelrys Software Inc. - processing
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 750 MHz