BMRB Entry 18124

Name: Solution Structure of D. radiodurans RecQ HRDC domain 1
Published: 2014-04-23

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR18124

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of D. radiodurans RecQ HRDC domain 1

Deposition date:

2011-12-08

Original release date:

2014-04-23

Authors:

Cao, Chunyang; Dong, Yuhui; Liu, Shanshan

Citation:

Citation: Liu, Shanshan; Zhang, Wen; Gao, Zengqiang; Ming, Qianqian; Hou, Haifeng; Lan, Wenxian; Wu, Houming; Cao, Chunyang; Dong, Yuhui. "NMR structure of the N-terminal-most HRDC1 domain of RecQ helicase from Deinococcus radiodurans." FEBS Lett. 587, 2635-2642 (2013).
PubMed: 23831579

Assembly members:

Assembly members:
D._radiodurans_RecQ, polymer, 75 residues, 8054.224 Da.

Natural source:

Natural source: Common Name: Deinococcus radiodurans Taxonomy ID: 1299 Superkingdom: Bacteria Kingdom: not available Genus/species: Deinococcus radiodurans

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
D._radiodurans_RecQ: HDAPLFEALRAWRLQKAKEL SLPPYTIFHDATLKTIAELR PGSHATLGTVSGVGGRKLAA YGDEVLQVVRDSSGG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	287
15N chemical shifts	71
1H chemical shifts	514

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	D. radiodurans RecQ	1

Entities:

Entity 1, D. radiodurans RecQ 75 residues - 8054.224 Da.

1

HIS

ASP

ALA

PRO

LEU

PHE

GLU

ALA

LEU

ARG

2

ALA

TRP

ARG

LEU

GLN

LYS

ALA

LYS

GLU

LEU

3

SER

LEU

PRO

TYR

THR

ILE

PHE

HIS

ASP

4

ALA

THR

LEU

LYS

THR

ILE

ALA

GLU

LEU

ARG

5

PRO

GLY

SER

HIS

ALA

THR

LEU

GLY

THR

VAL

6

SER

GLY

VAL

GLY

ARG

LYS

LEU

ALA

7

TYR

GLY

ASP

GLU

VAL

LEU

GLN

VAL

ARG

8

ASP

SER

GLY

Samples:

sample_1: D._radiodurans_RecQ, [U-100% 13C; U-100% 15N], 2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 760 mmHg; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	19317
PDB	2MA1