BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18157

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the Avirulence homolog-5   PubMed: 23075041

Deposition date: 2011-12-20 Original release date: 2012-01-04

Authors: Azurmendi, Hugo; Sun, Furong; Capelluto, Daniel

Citation: Sun, Furong; Kale, Shiv; Azurmendi, Hugo; Li, Dan; Tyler, Brett; Capelluto, Daniel. "Structural basis for interactions of the Phytophthora sojae RxLR effector Avh5 with phosphatidylinositol 3-phosphate and for host cell entry."  Mol. Plant Microbe Interact. 26, 330-344 (2013).

Assembly members:
Avh5, polymer, 121 residues, 13874 Da.

Natural source:   Common Name: Phytophthora sojae   Taxonomy ID: 67593   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Phytophthora sojae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX6P-1

Entity Sequences (FASTA):
Avh5: GPLGSTRVPDDANLQSVNAP VQTVTRSRRFLRTADTDIVY EPKVHNPGKKQVFIEDKLQK ALTDPKKNKKLYARWYNSGF TVKQVEGGLDQNENRELELT YKNLALGYAKYYQARRSQEA K

Data sets:
Data typeCount
13C chemical shifts323
15N chemical shifts103
1H chemical shifts103

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Avh51

Entities:

Entity 1, Avh5 121 residues - 13874 Da.

Residues 1-5 represent the residues from the vector

1   GLYPROLEUGLYSERTHRARGVALPROASP
2   ASPALAASNLEUGLNSERVALASNALAPRO
3   VALGLNTHRVALTHRARGSERARGARGPHE
4   LEUARGTHRALAASPTHRASPILEVALTYR
5   GLUPROLYSVALHISASNPROGLYLYSLYS
6   GLNVALPHEILEGLUASPLYSLEUGLNLYS
7   ALALEUTHRASPPROLYSLYSASNLYSLYS
8   LEUTYRALAARGTRPTYRASNSERGLYPHE
9   THRVALLYSGLNVALGLUGLYGLYLEUASP
10   GLNASNGLUASNARGGLULEUGLULEUTHR
11   TYRLYSASNLEUALALEUGLYTYRALALYS
12   TYRTYRGLNALAARGARGSERGLNGLUALA
13   LYS

Samples:

sample_1: Avh5, [U-100% 13C; U-100% 15N], 1 mM

sample_conditions_1: ionic strength: 100 mM; pH: 5.8; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

Topspin v2.1, CCPN, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, collection, processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

GB AEK80452 AEK80453 AEK80454 EGZ14529
REF XP_009528278

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts