BMRB Entry 18240
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18240
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Title: Mutant of the sub-genomic promoter from Brome Mosaic Virus PubMed: 22393035
Deposition date: 2012-02-06 Original release date: 2012-03-13
Authors: Skov, Joan
Citation: Skov, Joan; Gaudin, Mathieu; Podbevsek, Peter; Olsthoorn, Rene; Petersen, Michael. "The subgenomic promoter of brome mosaic virus folds into a stem-loop structure capped by a pseudo-triloop that is structurally similar to the triloop of the genomic promoter." RNA 18, 992-1000 (2012).
Assembly members:
RNA_(5'-R(*GP*AP*GP*GP*AP*CP*AP*UP*AP*GP*UP*CP*UP*UP*C)-3')_, polymer, 15 residues, 4792.932 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
RNA_(5'-R(*GP*AP*GP*GP*AP*CP*AP*UP*AP*GP*UP*CP*UP*UP*C)-3')_: GAGGACAUAGUCUUC
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 100 |
31P chemical shifts | 14 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | RNA (5'-R(*GP*AP*GP*GP*AP*CP*AP*UP*AP*GP*UP*CP*UP*UP*C)-3') | 1 |
Entities:
Entity 1, RNA (5'-R(*GP*AP*GP*GP*AP*CP*AP*UP*AP*GP*UP*CP*UP*UP*C)-3') 15 residues - 4792.932 Da.
1 | G | A | G | G | A | C | A | U | A | G | ||||
2 | U | C | U | U | C |
Samples:
sample_1: delA_RNA 2 mM; sodium phosphate 10 mM; sodium chloride 50 mM; EDTA 2 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 65 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR v3.851, Brunger - structure solution
NMR spectrometers:
- Varian INOVA 500 MHz