BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 18249

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18249

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Title: 1H, 13C and 15N backbone and side-chain resonance assignments of reduced CcmG from Escherichia coli   PubMed: 22585086

Deposition date: 2012-02-09 Original release date: 2013-02-20

Authors: Wu, Chengyan

Citation: Wu, Chengyan; Hong, Jing; Liao, Xinli; Guo, Chenyun; Wu, Xueji; Hu, Hongyu; Lin, Donghai. "(1)H, (13)C and (15)N backbone and side-chain resonance assignments of reduced CcmG from Escherichia coli."  Biomol. NMR Assignments 7, 105-108 (2013).

Assembly members:
CcmG, polymer, 175 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):
CcmG: ALLWQLARNAEGDDPTNLES ALIGKPVPKFRLESLDNPGQ FYQADVLTQGKPVLLNVWAT WCPTCRAEHQYLNQLSAQGI RVVGMNYKDDRQKAISWLKE LGNPYALSLFDGDGMLGLDL GVYGAPETFLIDGNGIIRYR HAGDLNPRVWEEEIKPLWEK YSKEAAQLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts638
15N chemical shifts156
1H chemical shifts965

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CcmG1

Entities:

Entity 1, CcmG 175 residues - Formula weight is not available

1   ALALEULEUTRPGLNLEUALAARGASNALA
2   GLUGLYASPASPPROTHRASNLEUGLUSER
3   ALALEUILEGLYLYSPROVALPROLYSPHE
4   ARGLEUGLUSERLEUASPASNPROGLYGLN
5   PHETYRGLNALAASPVALLEUTHRGLNGLY
6   LYSPROVALLEULEUASNVALTRPALATHR
7   TRPCYSPROTHRCYSARGALAGLUHISGLN
8   TYRLEUASNGLNLEUSERALAGLNGLYILE
9   ARGVALVALGLYMETASNTYRLYSASPASP
10   ARGGLNLYSALAILESERTRPLEULYSGLU
11   LEUGLYASNPROTYRALALEUSERLEUPHE
12   ASPGLYASPGLYMETLEUGLYLEUASPLEU
13   GLYVALTYRGLYALAPROGLUTHRPHELEU
14   ILEASPGLYASNGLYILEILEARGTYRARG
15   HISALAGLYASPLEUASNPROARGVALTRP
16   GLUGLUGLUILELYSPROLEUTRPGLULYS
17   TYRSERLYSGLUALAALAGLNLEUGLUHIS
18   HISHISHISHISHIS

Samples:

sample_1: CcmG, [U-100% 13C; U-100% 15N], 1 mM; DTT 10 mM; D2O 10%; H2O 90%; sodium phosphate 20 mM; sodium chloride 100 mM

sample_2: CcmG, [U-100% 13C; U-100% 15N], 1 mM; DTT 10 mM; D2O 100%; sodium phosphate 20 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAB36507 BAE76658 BAG77987 BAI26333 BAI31441
EMBL CAP76697 CAQ32598 CAQ89786 CAQ99122 CAR03623
GB AAA16387 AAC75255 AAG57330 AAN43798 AAN81186
REF NP_311111 NP_416699 NP_708091 WP_000824435 WP_000824436
SP P0AA86 P0AA87 P0AA88
AlphaFold P0AA86 P0AA87 P0AA88

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts