BMRB Entry 18251

Name: Cdc42Hs-GMPPCP Complex
Published: 2019-09-03

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18251

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Cdc42Hs-GMPPCP Complex

Deposition date:

2012-02-09

Original release date:

2019-09-03

Authors:

Moorman, Veroncia; Dogan, Jakob; Love, Fiona; Valentine, Kathleen; Wand, Joshua

Citation:

Citation: Moorman, Veronica; Valentine, Kathleen; Bedard, Sabrina; Kasinath, Vignesh; Dogan, Jakob; Love, Fiona; Wand, A Joshua. "Dynamic and thermodynamic response of the Ras protein Cdc42Hs upon association with the effector domain of PAK3" J. Mol. Biol. 426, 3520-3538 (2014).
PubMed: 25109462

Assembly members:

Assembly members:
Cdc42Hs, polymer, 180 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cdc42Hs: GSMQTIKCVVVGDGAVGKTC LLISYTTNKFPSEYVPTVFD NYAVTVMIGGEPYTLGLFDT AGQEDYDRLRPLSYPQTDVF LVCFSVVSPSSFENVKEKWV PEITHHCPKTPFLLVGTQID LRDDPSTIEKLAKNKQKPIT PETAEKLARDLKAVKYVECS ALTQKGLKNVFDEAILAALE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
order_parameters
- backbone_parameters
- methyl_parameters

Data type	Count
13C chemical shifts	219
15N chemical shifts	142
1H chemical shifts	436
order parameters	224

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Cdc42Hs	1

Entities:

Entity 1, Cdc42Hs 180 residues - Formula weight is not available

1	GLY	SER	MET	GLN	THR	ILE	LYS	CYS	VAL	VAL
2	VAL	GLY	ASP	GLY	ALA	VAL	GLY	LYS	THR	CYS
3	LEU	LEU	ILE	SER	TYR	THR	THR	ASN	LYS	PHE
4	PRO	SER	GLU	TYR	VAL	PRO	THR	VAL	PHE	ASP
5	ASN	TYR	ALA	VAL	THR	VAL	MET	ILE	GLY	GLY
6	GLU	PRO	TYR	THR	LEU	GLY	LEU	PHE	ASP	THR
7	ALA	GLY	GLN	GLU	ASP	TYR	ASP	ARG	LEU	ARG
8	PRO	LEU	SER	TYR	PRO	GLN	THR	ASP	VAL	PHE
9	LEU	VAL	CYS	PHE	SER	VAL	VAL	SER	PRO	SER
10	SER	PHE	GLU	ASN	VAL	LYS	GLU	LYS	TRP	VAL
11	PRO	GLU	ILE	THR	HIS	HIS	CYS	PRO	LYS	THR
12	PRO	PHE	LEU	LEU	VAL	GLY	THR	GLN	ILE	ASP
13	LEU	ARG	ASP	ASP	PRO	SER	THR	ILE	GLU	LYS
14	LEU	ALA	LYS	ASN	LYS	GLN	LYS	PRO	ILE	THR
15	PRO	GLU	THR	ALA	GLU	LYS	LEU	ALA	ARG	ASP
16	LEU	LYS	ALA	VAL	LYS	TYR	VAL	GLU	CYS	SER
17	ALA	LEU	THR	GLN	LYS	GLY	LEU	LYS	ASN	VAL
18	PHE	ASP	GLU	ALA	ILE	LEU	ALA	ALA	LEU	GLU

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D H(C)CH-COSY	sample_1	isotropic	sample_conditions_1
3D H(C)CH-TOCSY	sample_1	isotropic	sample_conditions_1
3D methyl (H)CCH-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_4	isotropic	sample_conditions_1
T1 N-H relaxation	sample_2	isotropic	sample_conditions_1
T1 N-H relaxation	sample_2	isotropic	sample_conditions_1
T2 N-H relaxation	sample_2	isotropic	sample_conditions_1
T2 N-H relaxation	sample_2	isotropic	sample_conditions_1
NOE N-H relaxation	sample_2	isotropic	sample_conditions_1
NOE N-H relaxation	sample_2	isotropic	sample_conditions_1
T1 CHD2 relaxation	sample_3	isotropic	sample_conditions_1
T1 CHD2 relaxation	sample_3	isotropic	sample_conditions_1
T1rho CHD2 relaxation	sample_3	isotropic	sample_conditions_1
T1rho CHD2 relaxation	sample_3	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 18251

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	GLY	SER	MET	GLN	THR	ILE	LYS	CYS	VAL	VAL
2	VAL	GLY	ASP	GLY	ALA	VAL	GLY	LYS	THR	CYS
3	LEU	LEU	ILE	SER	TYR	THR	THR	ASN	LYS	PHE
4	PRO	SER	GLU	TYR	VAL	PRO	THR	VAL	PHE	ASP
5	ASN	TYR	ALA	VAL	THR	VAL	MET	ILE	GLY	GLY
6	GLU	PRO	TYR	THR	LEU	GLY	LEU	PHE	ASP	THR
7	ALA	GLY	GLN	GLU	ASP	TYR	ASP	ARG	LEU	ARG
8	PRO	LEU	SER	TYR	PRO	GLN	THR	ASP	VAL	PHE
9	LEU	VAL	CYS	PHE	SER	VAL	VAL	SER	PRO	SER
10	SER	PHE	GLU	ASN	VAL	LYS	GLU	LYS	TRP	VAL
11	PRO	GLU	ILE	THR	HIS	HIS	CYS	PRO	LYS	THR
12	PRO	PHE	LEU	LEU	VAL	GLY	THR	GLN	ILE	ASP
13	LEU	ARG	ASP	ASP	PRO	SER	THR	ILE	GLU	LYS
14	LEU	ALA	LYS	ASN	LYS	GLN	LYS	PRO	ILE	THR
15	PRO	GLU	THR	ALA	GLU	LYS	LEU	ALA	ARG	ASP
16	LEU	LYS	ALA	VAL	LYS	TYR	VAL	GLU	CYS	SER
17	ALA	LEU	THR	GLN	LYS	GLY	LEU	LYS	ASN	VAL
18	PHE	ASP	GLU	ALA	ILE	LEU	ALA	ALA	LEU	GLU

1	GLY	SER	MET	GLN	THR	ILE	LYS	CYS	VAL	VAL
2	VAL	GLY	ASP	GLY	ALA	VAL	GLY	LYS	THR	CYS
3	LEU	LEU	ILE	SER	TYR	THR	THR	ASN	LYS	PHE
4	PRO	SER	GLU	TYR	VAL	PRO	THR	VAL	PHE	ASP
5	ASN	TYR	ALA	VAL	THR	VAL	MET	ILE	GLY	GLY
6	GLU	PRO	TYR	THR	LEU	GLY	LEU	PHE	ASP	THR
7	ALA	GLY	GLN	GLU	ASP	TYR	ASP	ARG	LEU	ARG
8	PRO	LEU	SER	TYR	PRO	GLN	THR	ASP	VAL	PHE
9	LEU	VAL	CYS	PHE	SER	VAL	VAL	SER	PRO	SER
10	SER	PHE	GLU	ASN	VAL	LYS	GLU	LYS	TRP	VAL
11	PRO	GLU	ILE	THR	HIS	HIS	CYS	PRO	LYS	THR
12	PRO	PHE	LEU	LEU	VAL	GLY	THR	GLN	ILE	ASP
13	LEU	ARG	ASP	ASP	PRO	SER	THR	ILE	GLU	LYS
14	LEU	ALA	LYS	ASN	LYS	GLN	LYS	PRO	ILE	THR
15	PRO	GLU	THR	ALA	GLU	LYS	LEU	ALA	ARG	ASP
16	LEU	LYS	ALA	VAL	LYS	TYR	VAL	GLU	CYS	SER
17	ALA	LEU	THR	GLN	LYS	GLY	LEU	LYS	ASN	VAL
18	PHE	ASP	GLU	ALA	ILE	LEU	ALA	ALA	LEU	GLU

1	GLY	SER	MET	GLN	THR	ILE	LYS	CYS	VAL	VAL
2	VAL	GLY	ASP	GLY	ALA	VAL	GLY	LYS	THR	CYS
3	LEU	LEU	ILE	SER	TYR	THR	THR	ASN	LYS	PHE
4	PRO	SER	GLU	TYR	VAL	PRO	THR	VAL	PHE	ASP
5	ASN	TYR	ALA	VAL	THR	VAL	MET	ILE	GLY	GLY
6	GLU	PRO	TYR	THR	LEU	GLY	LEU	PHE	ASP	THR
7	ALA	GLY	GLN	GLU	ASP	TYR	ASP	ARG	LEU	ARG
8	PRO	LEU	SER	TYR	PRO	GLN	THR	ASP	VAL	PHE
9	LEU	VAL	CYS	PHE	SER	VAL	VAL	SER	PRO	SER
10	SER	PHE	GLU	ASN	VAL	LYS	GLU	LYS	TRP	VAL
11	PRO	GLU	ILE	THR	HIS	HIS	CYS	PRO	LYS	THR
12	PRO	PHE	LEU	LEU	VAL	GLY	THR	GLN	ILE	ASP
13	LEU	ARG	ASP	ASP	PRO	SER	THR	ILE	GLU	LYS
14	LEU	ALA	LYS	ASN	LYS	GLN	LYS	PRO	ILE	THR
15	PRO	GLU	THR	ALA	GLU	LYS	LEU	ALA	ARG	ASP
16	LEU	LYS	ALA	VAL	LYS	TYR	VAL	GLU	CYS	SER
17	ALA	LEU	THR	GLN	LYS	GLY	LEU	LYS	ASN	VAL
18	PHE	ASP	GLU	ALA	ILE	LEU	ALA	ALA	LEU	GLU