BMRB Entry 18265
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18265
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Title: WIP C-terminal domain PubMed: 23870269
Deposition date: 2012-02-15 Original release date: 2019-08-29
Authors: Novacek, Jiri; Haba, Noam; Shaked, Hadassa; Chill, Jordan; Zidek, Lukas; Sklenar, Vladimir
Citation: Haba, Noam; Gross, Renana; Novacek, Jiri; Shaked, Hadassa; Zidek, Lukas; Barda-Saad, Mira; Chill, Jordan. "NMR determines transient structure and dynamics in the disordered C-terminal domain of WASp interacting protein." Biophys. J. 105, 481-493 (2013).
Assembly members:
WIPc, polymer, 114 residues, 12841.0 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
WIPc: GSSHHHHHHVDSPRSGPRPP
LPPDRPSAGAPPPPPPSTSI
RNGFQDSPCEDEWESRFYFH
PISDLPPPEPYVQTTKSYPS
KLARNESRSSSNRRERGAPP
LPPIPRLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 325 |
| 15N chemical shifts | 111 |
| 1H chemical shifts | 316 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | WIPc | 1 |
Entities:
Entity 1, WIPc 114 residues - 12841.0 Da.
| 1 | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | VAL | ||||
| 2 | ASP | SER | PRO | ARG | SER | GLY | PRO | ARG | PRO | PRO | ||||
| 3 | LEU | PRO | PRO | ASP | ARG | PRO | SER | ALA | GLY | ALA | ||||
| 4 | PRO | PRO | PRO | PRO | PRO | PRO | SER | THR | SER | ILE | ||||
| 5 | ARG | ASN | GLY | PHE | GLN | ASP | SER | PRO | CYS | GLU | ||||
| 6 | ASP | GLU | TRP | GLU | SER | ARG | PHE | TYR | PHE | HIS | ||||
| 7 | PRO | ILE | SER | ASP | LEU | PRO | PRO | PRO | GLU | PRO | ||||
| 8 | TYR | VAL | GLN | THR | THR | LYS | SER | TYR | PRO | SER | ||||
| 9 | LYS | LEU | ALA | ARG | ASN | GLU | SER | ARG | SER | GLY | ||||
| 10 | SER | ASN | ARG | ARG | GLU | ARG | GLY | ALA | PRO | PRO | ||||
| 11 | LEU | PRO | PRO | ILE | PRO | ARG | LEU | GLU | HIS | HIS | ||||
| 12 | HIS | HIS | HIS | HIS |
Samples:
sample_1: WIPc, [U-98% 13C; U-98% 15N], 1.0 ± 0.03 mM; potassium phosphate 20 mM; sodium chloride 20 mM; beta-mercaptoethanol 10 mM; D2O, [U-99% 2H], 10%; H2O 90%
sample_conditions_1: ionic strength: 0.06 M; pH: 5.1; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| CON | sample_1 | isotropic | sample_conditions_1 |
| 3D C_CANCO | sample_1 | isotropic | sample_conditions_1 |
| 3D C_CBCACON | sample_1 | isotropic | sample_conditions_1 |
| 3D C_CBCANCO | sample_1 | isotropic | sample_conditions_1 |
| 5D C_CACONCACO | sample_1 | isotropic | sample_conditions_1 |
| 5D C_NCOCANCO | sample_1 | isotropic | sample_conditions_1 |
| 4D C_HabCabCON | sample_1 | isotropic | sample_conditions_1 |
| 4D C_HabCabNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.1, Bruker Biospin, Goddard - collection, peak picking, processing
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 700 MHz