BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18398

Title: Solution Structure of the WNK1 Autoinhibitory Domain   PubMed: 23376100

Deposition date: 2012-04-13 Original release date: 2012-04-24

Authors: Moon, Thomas; Correa, Fernando; Gardner, Kevin; Goldsmith, Elizabeth

Citation: Moon, Thomas; Correa, Fernando; Kinch, Lisa; Piala, Alexander; Gardner, Kevin; Goldsmith, Elizabeth. "Solution Structure of the WNK1 Autoinhibitory Domain, a WNK-Specific PF2 Domain."  J. Mol. Biol. 425, 1245-1252 (2013).

Assembly members:
WNKAI, polymer, 98 residues, 11358.971 Da.

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHis-parallel

Entity Sequences (FASTA):
WNKAI: GAMDPQEETGVRVELAEEDD GEKIAIKLWLRIEDIKKLKG KYKDNEAIEFSFDLERDVPE DVAQEMVESGYVCEGDHKTM AKAIKDRVSLIKRKREQR

Data sets:
Data typeCount
13C chemical shifts438
15N chemical shifts97
1H chemical shifts704

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1WNK1 Autoinhibitory Domain1

Entities:

Entity 1, WNK1 Autoinhibitory Domain 98 residues - 11358.971 Da.

1   GLYALAMETASPPROGLNGLUGLUTHRGLY
2   VALARGVALGLULEUALAGLUGLUASPASP
3   GLYGLULYSILEALAILELYSLEUTRPLEU
4   ARGILEGLUASPILELYSLYSLEULYSGLY
5   LYSTYRLYSASPASNGLUALAILEGLUPHE
6   SERPHEASPLEUGLUARGASPVALPROGLU
7   ASPVALALAGLNGLUMETVALGLUSERGLY
8   TYRVALCYSGLUGLYASPHISLYSTHRMET
9   ALALYSALAILELYSASPARGVALSERLEU
10   ILELYSARGLYSARGGLUGLNARG

Samples:

sample_1: WNKAI, [U-100% 13C; U-100% 15N], 0.65 mM

sample_conditions_1: ionic strength: 0.010 M; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

ARIA, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAA20802 BAD32213 BAE21813
EMBL CAC15059
GB AAF31483 AAF74258 AAH30370 AAI28629 AAI38446
REF NP_001002823 NP_001090703 NP_001171914 NP_001171949 NP_001171950
SP P83741 Q9H4A3 Q9JIH7
TPG DAA29175
AlphaFold Q9JIH7 P83741 Q9H4A3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts