BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18405

Title: Structure of Sviceucin, an type I lasso peptide from Streptomyces sviceus

Deposition date: 2012-04-18 Original release date: 2012-08-30

Authors: Li, Yanyan; Ducasse, Remi; Blond, Alain; Zirah, Severine; Goulard, Christophe; Lescop, Ewen; Guittet, Eric; Pernodet, Jean-Luc; Rebuffat, Sylvie

Citation: Li, Yanyan; Ducasse, Remi; Blond, Alain; Zirah, Severine; Goulard, Christophe; Lescop, Ewen; Guittet, Eric; Pernodet, Jean-Luc; Rebuffat, Sylvie. "Structure and biosynthesis of Sviceucin, an type I lasso peptide from Streptomyces sviceus"  .

Assembly members:
entity, polymer, 20 residues, 2106.426 Da.

Natural source:   Common Name: high GC Gram+   Taxonomy ID: 285530   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomyces sviceus

Experimental source:   Production method: recombinant technology   Host organism: Streptomyces coelicolor   Vector: pWED4

Entity Sequences (FASTA):
entity: CVWGGDCTDFLGCGTAWICV

Data sets:
Data typeCount
13C chemical shifts59
1H chemical shifts119

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sviceucin1

Entities:

Entity 1, Sviceucin 20 residues - 2106.426 Da.

1   CYSVALTRPGLYGLYASPCYSTHRASPPHE
2   LEUGLYCYSGLYTHRALATRPILECYSVAL

Samples:

sample_1: Sviceucin 2.4 mM; Methanol 2.4 mM

sample_conditions_1: pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, CCPN, Neidig, Geyer, Gorler, Antz, Saffrich, Beneicke, Kalbitzer - data analysis, processing, structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB EDY58505